[关键词]
[摘要]
目的 研究半边旗Pteris semipinnata的化学成分。方法 综合应用硅胶、Sephadex LH-20凝胶、ODS、大孔树脂、MCI Gel柱色谱和半制备高效液相色谱法分离纯化,并通过NMR和HR-ESI-MS法鉴定结构。结果 从半边旗全草甲醇提取物中共分离20个化合物,分别鉴定为芹菜素(1)、芹菜素-7-O-β-D-吡喃葡萄糖苷(2)、芹菜素-7-O-β-D-龙胆二糖苷(3)、芹菜素-7-O-β-D-吡喃葡萄糖-4'-O-α-L-吡喃鼠李糖苷(4)、异佛莱心苷(5)、木犀草素(6)、木犀草苷(7)、木犀草素-7-O-β-D-龙胆二糖苷(8)、槲皮素-3-O-β-D-吡喃葡萄糖苷(9)、山柰酚-3-O-β-D-吡喃葡萄糖苷(10)、芦丁(11)、表没食子儿茶素(12)、松脂素-4-O-β-D-吡喃葡萄糖苷(13)、连翘苷(14)、岩白菜素(15)、原儿茶酸(16)、反式咖啡酸(17)、没食子酸(18)、β-谷甾醇(19)、β-胡萝卜苷(20)。结论 化合物3、5、12、13、15~18为首次从该属植物中分离得到,化合物2~5、8~13、15~20为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents from the whole plants of Pteris semipinnata. Methods The chemical constituents were isolated by silica gel, sephadex LH-20, ODS, macroporous resin, MCI Gel resin and semi-preparation HPLC. Their structures were determined on the basis of NMR and MS analysis. Results Twenty compounds were isolated and identified as apigenin (1), apigenin-7-O-β-D-glucopyranoside (2), apigenin-7-O-β-D-gentiobioside (3), apigenin-7-O-β-D-glucopyranosyl-4'-O-α-L-rhamnopyranoside (4), isoviolanthin (5), luteolin (6), luteoloside (7), luteolin-7-O-β-D-gentiobioside (8), quercetin-3-O-β-D-glucopyranoside (9), kaempferol-3-O-β-D-glucopyranoside (10), rutin (11), epigallocatechin (12), pinoresinol-4-O-β-D-glucopyranoside (13), phillyrin (14), bergeninum (15), protocatechuic acid (16), trans-caffeic acid (17), gallic acid (18), β-sitosterol (19), and β-daucosterol (20). Conclusion Compounds 3, 5, 12, 13, and 15-18 are isolated from the plants of Pteris, and 2-5, 8-13, and 15-20 are isolated from this plant for the first time.
[中图分类号]
[基金项目]
广东省高等学校优秀青年教师培养计划(Yq2013045)