目的 研究不同预处理方式及培养温度对北柴胡种子萌发和幼苗生长的影响，为北柴胡人工栽培提供科学依据。方法 测定种子千粒质量、净度、含水量及吸水率，采用双层滤纸培养法，将预处理后的种子置于20℃恒温40%光照的培养箱中培养，不同培养温度处理组蒸馏水浸泡24 h后在对应温度的培养箱中培养，观察种子发芽势、发芽率、发芽指数、活力指数和幼苗根长及苗高的变化。结果 种子净度约91.51%，千粒质量为（2.83±0.03）g，吸水率为自然风干种子质量的2.24倍，含水量约7.3%。不同激素对种子的处理效果不同，其中0.6 mg/L 6-BA处理效果最好；水浴能普遍提高幼苗活力指数，40℃水浴显著促进了北柴胡种子萌发，幼苗生长；15℃环境下种子的萌发率最高，但幼苗根长、苗高较低。结论 北柴胡种子预处理方式为浸泡在0.6 mg/L 6-BA中，40℃水浴2 h，初始培养温度为15℃，萌发后移至20℃下继续培养，可有效提高种子萌发率及成苗率，对北柴胡的种子育苗及人工栽培具有重要指导意义。

Objective To study the effects of different pretreatment methods and culture temperatures on Seed Germination and seedling growth of Bupleurum chinense, in order to provide scientific basis for artificial cultivation of B. chinense. Methods Several physiological indexes such as the weight, pure rate, content of moisture, the rate of water absorption per thousand seeds were measured. Adopting a double-layer filter paper culture method, the pretreated seeds were cultured in the incubator at 20℃ in the 40% light. In different culture temperature treatment groups, the seeds were soaked in distilled water 24 h and then cultured in the corresponding temperature incubator. The germination energy, germination percentage, germination index, vigor index of seeds, and the root length and the shoot height of the seedling were recorded. Results The pure rate of seeds was 91.51%, the weight of one thousand seeds was (2.83 ±0.03) g and the weight of seed after absorbing water was about 2.24 times more than the weight of naturally dried seed. The content of moisture of seeds was 7.3%. Different hormones had different effects on seeds, among them the effect of 0.6 mg/L 6-BA treatment was the best. Water bath could increase the seedling vigor index, and 40℃ of its temperature significantly promoted the seed germination and seedling growth. The germination rate of seeds was the highest under the condition of 15℃, but the root length and seedling height were lower. Conclusion The suitable condition for seed pretreatment and culture temperature of B. chinense established in this research can effectively improve the germination rate and seedling rate, which is of great significance for seed breeding and artificial cultivation of B. chinense.

甘草等7种中药饮片标准化建设（ZYBZH-Y-JL-25）