[关键词]
[摘要]
目的 研究冷蒿Artemisia frigida全草的化学成分,并初步筛选其药理活性。方法 应用多种色谱技术对冷蒿全草的粗提取物进行系统的分离纯化,采用光谱学和波谱学方法鉴定化合物结构,并对部分单体化合物进行过氧化物酶体增殖物激活受体γ(PPARγ)激动活性及蛋白酪氨酸磷酸酯酶1B(PTP1B)抑制活性的筛选。结果 从冷蒿全草提取物中分离得到了26个化合物,分别鉴定为柳穿鱼黄素(1)、棕矢车菊素(2)、金圣草黄素(3)、麦黄酮(4)、3-羰基-吉玛-1(10),11(13)-二烯-6α,12-内酯(5)、蓍素(6)、1,10β-环氧蓍素(7)、滨蒿内酯(8)、4-羟基苯乙酮(9)、猫眼草黄素(10)、棕鳞矢车菊黄酮素(11)、11α,13-二氢魃蒿内酯(12)、chrysanthemin A(13)、异泽兰黄素(14)、泽兰黄素(15)、南艾蒿烯内酯(16)、6-甲氧基麦黄酮(17)、hanphyllin(18)、蓟黄素(19)、利得亭(20)、desacetylmatricarin(21)、subchrysine(22)、木犀草素(23)、咖啡酸(24)、藿香苷(25)、田蓟苷(26)。结论 化合物5、7、11、18、25、26 为首次从蒿属植物中分离得到,化合物10、12、13、16、17、22 为首次从该植物中分离得到;化合物2 和15 有较弱的PPARγ激动活性,化合物1 和3 对PTP1B有中等强度的抑制作用。
[Key word]
[Abstract]
Objective To study the chemical constituents from plant of Artemisia frigida Willd. and its pharmacological activities. Methods The compounds were isolated and purified by various chromatography methods. All compounds were identified by spectroscopic and chromatographic techniques, and were screening for their PPARγ activating activity and PTP1B inhibitory activity. Results There were 26 compounds isolated from the plant of A. frigida and identified as pectolinarigenin (1), jaceosidin (2), chrysoeriol (3), tricin (4), 3-oxogermacra-1(10),11(13)-dien-6α,12-olide (5), achillin (6), 1,10β-epoxyachillin (7), scoparone (8), 4-hydroxyacetophenone (9), chrysosplentin (10), jaceidin (11), 11α,13-dihydroyomogin (12), chrysanthemin A (13), eupatilin (14), eupatrin (15), artemorin (16), 6-methoxytricin (17), hanphyllin (18), cirsimaritin (19), ridentin (20), desacetylmatricarin (21), subchrysine (22), luteolin (23), caffeic acid (24), agastachoside (25), and tilianin (26). Conclusion Compounds 5, 7, 11, 18, 25, and 26 are firstly isolated from the Artemisia. Compounds 10, 12, 13, 16, 17, and 22 are firstly isolated from this plant. Compounds 2 and 15 exhibited weak activity of PPARγ. Compounds 1 and 3 had inhibitory effect on PTP1B.
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[基金项目]
国家自然科学基金资助项目(81302664);河北省教育厅重点课题(ZD2016093);河北省中医药管理局课题(2016040)