目的 探讨黄芩素对雷公藤甲素诱导小鼠卵母细胞损伤的保护作用。方法 健康雌性BALB/C小鼠随机分为对照组，模型组，黄芩素低、高剂量组。对照组ig含5%二甲基亚砜的生理盐水，模型组ig 25 μg/kg雷公藤甲素，黄芩素低、高剂量组分别ig 25 μg/kg雷公藤甲素＋100 mg/kg黄芩素、25 μg/kg雷公藤甲素＋500 mg/kg黄芩素，共给药50 d。实验第41天起，连续阴道涂片，至第47天，进行超排卵实验。第50天处死各组小鼠，收集计数卵子。留取卵巢进行苏木精-伊红（HE）染色检测卵巢病理，计数各级卵泡。测定各组小鼠卵巢组织中超氧化物歧化酶（SOD）活性，谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）的量，评价氧化应激水平。Western blotting法检测小鼠卵母细胞中氧化还原相关蛋白抗硫氧还蛋白还原酶（TrxR）、硫氧还蛋白（Trx）、谷胱甘肽还原酶（GR）、GSH-Px的表达量。结果 雷公藤甲素显著引起小鼠卵巢损伤，表现为卵泡数量显著减少，卵巢间质区域结缔组织疏松；并使SOD活性降低，GSH-Px水平降低，MDA水平增加；黄芩素组小鼠卵巢发育较模型组有明显改善，卵泡细胞数量增多，间质区域结缔组织饱满。卵巢组织中SOD活性升高，GSH-Px水平升高，MDA水平降低。Western blotting结果显示，与对照组比较，模型组小鼠卵母细胞中的TrxR表达量明显下降；与模型组比较，黄芩素组小鼠卵母细胞中TrxR的表达量显著上升。结论 黄芩素可通过上调TrxR表达量提高小鼠卵巢组织的抗氧化能力，对雷公藤甲素诱导的卵巢损伤起到保护作用。

Objective To investigate the effect of baicalein on mouse ovarian injury induced by triptolide. Methods Female healthy BALB/C mice were randomized into four groups. The mice in control group were given water contained 5% DMSO. The mice in model group were given 25 μg/kg triptolide orally. While the mice in baicalein treated groups were given 25 μg/kg triptolide plus 100 mg/kg and 500 mg/kg baicalein, respectively. On the 41st d of experiment, continuous vaginal smear were made to measure the sexual cycle of mice. On the 47th d, the superovulation experiment was carried out. On the 50th d, the mice were sacrificed to collect the ovaries. Ovaries were examined by hematoxylin and eosin (HE) staining, and the levels of follicles were counted. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in the ovarian tissue of each group were measured to evaluate the level of oxidative stress. Western blotting assay was performed to measure the expression levels of thioredoxin reductase (TrxR), thioredoxin (Trx), glutathione reductase (GR), and GSH-Px. Results Triptolide significantly induced ovarian damage, reduced the number of follicles, induced ovarian interstitial connective tissue regional osteoporosis, and decreased the activities of SOD and GSH-Px, while increased the content of MDA. On the contrary, when adding baicalein, the mice ovary development was obviously improved. The follicle cell number increased, and the connective tissue in interstitial region of ovary was well-stacked. The activity of SOD and GSH-Px in ovarian tissue was increased and the content of MDA was decreased. Western Blotting results showed that the expression levels of TrxR in model group mice were significantly down-regulated compared with those of control group. However, compared with control group, the expression levels of TrxR were significantly up-regulated by baicalein treatment. Conclusion Baicalein can enhance the anti-oxidative ability of mouse ovarian tissue by up-regulating TrxR expression, and may play an important role in the protection of ovarian injury induced by triptolide.