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[摘要]
目的 研究绵马贯众Dryopteris crassirhizoma中间苯三酚类化合物并探讨其抑菌活性。方法 采用硅胶、Sephadex LH-20、半制备液相及重结晶等方法进行分离纯化,根据理化性质和波谱数据(MS、1H-NMR、13C-NMR)鉴定结构;采用美国临床实验室标准研究所(CLSI)制定的M38-A2和M07-A9方案分别研究化合物对真菌和细菌的抑菌活性。结果 从绵马贯众50%乙醇提取物中分离得到10个间苯三酚类化合物,分别鉴定为1-丁酰基间苯三酚(1)、1-甲基-3-丁酰基间苯三酚(2)、2-乙酰基-4-丁酰基间苯三酚(3)、1-甲基-3-乙酰基-5-丁酰基间苯三酚(4)、黄绵马酸PB(5)、异黄绵马酸AB(6)、异黄绵马酸AB(6)、黄绵马酸AA(7)、绵马酸ABA(8)、黄绵马酸AB(9)、绵马酸ABP(10)。化合物8对金黄色葡萄球菌和表皮葡萄球菌表现出与阳性对照头孢西丁相当的抑制活性,最小抑菌浓度(MIC)均达到2.5μg/mL。结论 化合物1~4首次从绵马贯众中分离得到。化合物8对真菌和细菌都有明显的抑菌活性。
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[Abstract]
Objective To isolate the phloroglucinol derivatives in Dryopteris crassirhizoma and to discuss its antibacterial activity. Methods The phloroglucinol derivatives were isolated and purified by column chromatography on silica gel, Sephadex LH-20, semi preparative liquid phase, and recrystallization. Structures were proved by physicochemical properties and spectral methods (MS, 1H-NMR, 13C-NMR). The antibacterial activities against fungi and bacteria were tested by CLSI M38-A2 and M07-A9. Results 10 derivatives were obtained from ethanol extracts of D. crassirhizoma, and were identified as 1-butyrylphloroglucinol (1), 1-methyl-3-butyrylphloroglucinol (2), 2-acetyl-4-butyrylphloroglucinol (3), 1-methyl-3-acetyl-5-butyrylphloroglucinol (4), flavaspidic acid PB (5), norflavaspidic acid AB (6), flavaspidic acid AA (7), filixic acid ABA (8), flavaspidic acid AB (9), and filixic acid ABP (10). The antibacterial activity of compound 8 against Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis) was similar to cefoxitin (positive control), and the MIC of compound 8 was 2.5 μg/mL. Conclusion Compounds 1-4, new compounds, are isolated from D. crassirhizoma. Compound 8 has both antibacterial activities against fungi and bacteria.
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[基金项目]
广东省科技厅应用型研发专项(2015B020234009);2015年国家中医药管理局中医药行业科研专项(201507004);广东省普通高校省级重大科研项目(2016KZDXM039);广东大学生科技创新培育专项(攀登计划51328004)