[关键词]
[摘要]
目的 研究长柱金花茶Camellia nitidissima var.longistyla叶的化学成分。方法 采用硅胶柱色谱、Sephadex LH-20、制备液相等多种手段进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果 从长柱金花茶叶95%乙醇提取物中分离得到16个化合物,分别鉴定为山柰酚(1)、3,5,6,7,4'-五羟基二氢黄酮(2)、6-羟基山柰酚(3)、(-)-表阿福豆素(4)、(-)-表儿茶素(5)、山柰酚-3-O-β-D-葡萄糖苷(6)、α-菠菜甾醇(7)、β-胡萝卜苷(8)、5,7,3',4'-四甲氧基黄酮(9)、7,3',4'-三甲氧基-5-羟基黄酮(10)、5,7,3',4',5'-五甲氧基黄酮(11)、3-O-乙酰齐墩果酸(12)、正三十四烷醇(13)、β-香树脂醇(14)、α-菠菜甾醇-3-O-β-D-吡喃半乳糖苷(15)、芦丁(16)。结论 所有化合物均为首次从该植物中分离得到,其中化合物2、3、9~11为首次从该属植物中分离得到。
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[Abstract]
Objective To investigate the chemical constituents in the root of Camellia nitidissima var. longistyla. Methods The chemical constituents were separated and purified by silica gel, sephadex LH-20 column chromatography, preparative HPLC and so on. Their structures were determined by physical constants and spectral analyses. Results Sixteen compounds were obtained and identified as kaempferol (1), 3,5,6,7,4'-pentahydroxyflavone (2), 6-hydroxykaempferol (3), (−)-epiafzelechin (4), (−)-epicatechin (5), kaempferol-3-O-β-D-glucopyranoside (6), α-spinasterol (7), β-daucosterol (8), 5,7,3',4'-tetramethoxyflavone (9), 7,3',4'-trimethoxy-5-hydroxyflavone (10), 5,7,3',4',5'-pentamethoxyflavone (11), 3-O-acetyl oleanolic acid (12), n-tetratriacontanol (13), β-amyrin (14), α-spinasterol-3-O-β-D-galactopyranoside (15), and rutin (16). Conclusion All compounds are isolated from the plant for the first time. Compounds 2, 3, and 9-11 are isolated from the plants of Camellia for the first time.
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[基金项目]
广西基础研究专项:金花茶及其制品质量控制方法研究(2015ACZ03);广西创新能力建设项目:金花茶生物活性成分分离纯化及其技术标准研究(11-114-14B)