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[摘要]
目的 研究青钱柳Cyclocarya paliurus叶子的化学成分。方法 采用色谱分离技术进行分离和纯化,并根据谱学数据鉴定化合物的结构。结果 从青钱柳叶子的甲醇提取物中分离得到14个化合物,包括11个黄酮类和3个酚酸类化合物,分别鉴定为山柰酚(1)、槲皮素(2)、芹菜素(3)、苜蓿素(4)、异泽兰黄素(5)、3,7,8,3'-tetrahydroxy-4'-methoxyflavone(6)、杨梅素(7)、3,5-dihydroxy-7-methoxy-3',4'-methylenedioxyflavone(8)、异槲皮苷(9)、槲皮素-3-O-β-D-葡萄糖醛酸苷(10)、杨梅素-3-O-β-D-葡萄糖醛酸苷(11)、咖啡酸(12)、5-咖啡酰基奎宁酸(13)和3-羟基苯甲酸-4-O-β-D-吡喃葡萄糖苷(14)。结论 化合物3~5和14为首次从青钱柳中分离得到,化合物6和8为首次从青钱柳属植物中分离得到。化合物1~11显示中等强度的细胞毒活性,化合物12~14显示显著的细胞毒活性。
[Key word]
[Abstract]
Objective To study the chemical constituents from the leaves of Cyclocarya paliurus. Methods The compounds were isolated and purified by chromatographic techniques and their structures were identified on the basis of spectral features. Results Forteen known compounds, including 11 flavonoids and three phenolic acids, named kaempferol (1), quercetin (2), apigenin (3), tricin (4), eupatilin (5), 3,7,8,3'-tetrahydroxy-4'-methoxyflavone (6), myricetin (7), 3,5-dihydroxy-7-methoxy-3',4'-methylenedioxyflavone (8), isoquercitrin (9), quercetin-3-O-β-D-glucuronide (10), myricetin-3-O-β-D-glucuronide (11), caffeic acid (12), 5-caffeoylquinic acid (13), and 3-hydroxybenzoic acid-4-O-β-D-glucopyranoside (14) were isolated from the leaves of C. paliurus. Conclusion Compounds 3, 4, 5, and 14 are firstly obtained from C. paliurus. Compounds 6 and 8 are isolated from the genus of Cyclocarya for the first time. Compounds 1-11 showed moderate active against the brine shrimp larvae, and compounds 12-14 indicated significant growth inhibitory activity against the brine shrimp (Artemia salina) larvae.
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[基金项目]
国家自然科学基金资助项目(21702187);浙江省农业生物资源生化制造协同创新中心开放基金项目(2016KF0025,2016KF0003,2016KF0007);浙江省重点科技创新团队项目(2013TD17);浙江省重点研发项目(2017C02012);浙江省自然科学基金项目(LQ15C020001)