[关键词]
[摘要]
目的 研究绿藻孔石莼Ulva pertusa的化学成分。方法 采用硅胶、ODS、Diaion HP-20、Sephadex LH-20等柱色谱和制备HPLC等技术进行分离纯化,根据理化性质和谱学数据鉴定化合物结构。结果 从孔石莼乙醇提取物中分离得到18个化合物,分别鉴定为异植物醇(1)、3-吲哚甲酸(2)、1-O-十六碳酰基-3-O-(6'-硫代-α-D-脱氧吡喃葡萄糖基)甘油(3)、(2S)-1-O-十六碳酰基-3-O-[α-D-吡喃半乳糖基-(1→2)-β-D-吡喃半乳糖基]甘油(4)、3-甲基亚砜基丙酸(5)、3-氯丙酸(6)、酪醇(7)、对羟基苯甲酸(8)、对羟基苯乙酸(9)、6-乙烯基己内酯(10)、黑麦草内酯(11)、向日葵香波龙大柱酮D(12)、壬二酸(13)、琥珀酸(14)、8-羟基-(6E)-辛烯酸(15)、3-乙氧基丙酸(16)、正丁基-β-D-吡喃果糖苷(17)、焦谷氨酸正丁酯(18)。结论 化合物1 ~16为首次从孔石莼中分离得到,化合物5、6、10、15和16为首次从天然产物中分离得到,化合物17和18为提取过程中产生的β-D-吡喃果糖苷和焦谷氨酸人工产物。
[Key word]
[Abstract]
Objective To investigate the chemical constituents from the ethanol extract of Ulva pertusa. Methods The compounds were isolated and purified by chromatography on silica gel, ODS, Diaion HP-20, Sephadex LH-20, and preparative HPLC methods. Their structures were elucidated on the basis of spectral data. Results Eighteen compounds were isolated and identified as isophitol (1), indole-3-carboxylic acid (2), 1-O-palmitoyl-3-O-(6'-sulfo-α-D-quinovopyranosyl) glycerol (3), (2S)-1-O-palmitoyl-3-O-[α-D-galactopyranosyl-(1→2)-β-D-galactopyranosyl] glycerol (4), 3-methylsulfoxypropionic acid (5), 3-chloropropionic acid (6), tyrosol (7), 4-hydroxybenzoic acid (8), 4-hydroxyphenylacetic acid (9), 6-vinyl hexanolide (10), loliolide (11), annuionone D (12), azelaic acid (13), succinic acid (14), 8-hydroxy-(6E)-octenoic acid (15), 3-ethoxypropionic acid (16), n-butyl β-D-fructopyranoside (17), and n-butyl pyroglutamate (18). Conclusion Compounds 1-16 are isolated from this alga for the first time, and compounds 5, 6, 10, 15, and 16 are obtained from natural products for the first time. Compounds 17 and 18 are artifacts of isolation from β-D-fructopyranoside and pyroglutamic acid.
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[基金项目]
国家自然科学基金资助项目(30672599)