[关键词]
[摘要]
目的 研究水栀子Gardenia jasminosides var.radicans的酚性成分。方法 采用硅胶、Toyopearl HW-40、Sephadex LH-20、MCI gel CHP-20、ODS柱色谱法及制备液相进行分离纯化,根据理化性质和波谱数据鉴定化合物结构。结果 从水栀子醋酸乙酯部位分离得到17个酚性化合物,分别鉴定为3,3',5-trimethoxy-9,9'-epoxylignane-4,4'-diol(1)、3,4-二香草酰基四氢呋喃(2)、marphenol C(3)、芥子醛(4)、对羟基肉桂酸(5)、异阿魏酸(6)、咖啡酸(7)、异秦皮啶(8)、异莨菪内酯(9)、6-甲氧基-7-羟基香豆素(10)、6,7-二羟基香豆素(11)、丁香酸(12)、3-羟基-4-甲氧基苯甲酸(13)、3,4-二羟基苯甲酸(14)、对羟基苯甲酸(15)、没食子酸-3-甲基醚(16)、没食子酸(17)。结论 以上化合物均为首次从水栀子中分离得到。
[Key word]
[Abstract]
Objective To investigate the phenolic constituents in the fruit of Gardenia jasminosides var. radicans. Methods The phenolic constituents were isolated and identified by chromatography on silica gel, Toyopearl HW-40, Sephadex LH-20, MCI gel CHP-20, ODS, and RP-HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral analyses. Results Seventeen compounds were isolated from 50% aqueous acetone extract from the fruit of Gardenia jasminosides var. radicans and identified as 3,3',5-trimethoxy-9,9'-epoxylignane-4,4'-diol (1), 3,4-divanillyltetrahydrofuran (2), marphenol C (3), sinapaldehyde (4), hydroxycinnamic acid (5), isoferulic acid (6), caffeic acid (7), isofraxidin (8), isoscopoletin (9), 6-methoxy-7-hydroxycoumarin (10), 6,7-dihydroxycoumarin (11), syringic acid (12), 3-hydroxy-4-methoxybenzoic acid (13), 3,4-dihydroxybenzoic acid (14), p-hydroxybenzoate (15), 4,5-dihydroxy-3-methoxy-benzoic acid (16), and gallic acid (17). Conclusion All 17 compounds are isolated from this plant for the first time.
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[基金项目]
教育部科学技术研究重点资助项目(DF2003078)