[关键词]
[摘要]
目的 研究霍山石斛内生真菌Fusarium lactis的次生代谢产物。方法 利用硅胶、SephadexLH-20、反相、中压、高效液相制备等多种色谱法分离纯化,根据波谱方法对化合物进行结构鉴定。结果 从霍山石斛内生真菌Fusarium lactis中分离得到21个化合物,分别鉴定为N-苯乙基乙酰胺(1)、1H-indole-3-carbaldehyde(2)、胸腺嘧啶(3)、尿嘧啶(4)、lignoren(5)、尿嘧啶核苷(6)、己六醇(7)、腺嘌呤核苷(8)、环-甘氨酸-(L)-脯氨酸(9)、环(D)-脯氨酸-(L)-苯丙氨酸(10)、环(L)-脯氨酸-(L)-苯丙氨酸(11)、2-pyrrolidinone(12)、N-methyl-2-pyrolidinone(13)、环(L)-4-羟基-脯氨酸-(L)-苯丙氨酸(14)、brevianamide F(15)、3-甲基哌嗪-2,5-二酮(16)、7,8-dimethylbenzo[g]pteridine-2,4(1H,3H)-dione(17),环(L)-酪氨酸-(L)-苯丙氨酸(18)、啤酒甾醇(19)、大豆黄素(20)、赤藓糖醇(21)。结论 所有化合物均为首次从霍山石斛内生真菌Fusarium lactis中分离得到。
[Key word]
[Abstract]
Objective To study the secondary metabolites of endophytic fungus Fusarium lactis in Dendrobium huoshanense. Methods Compounds were isolated from the EtOAc extract by chromatography technology and their structures were elucidated on the basis of comprehensive spectroscopic analysis. Results Twenty-one compounds were isolated and identified as N-phenethylacetamide (1), 1H-indole-3-carbaldehyde (2), thymidine (3), uracil (4), lignoren (5), uridine (6), hexahydrate (7), adenosine (8), cyclo-glycine-(L)-proline (9), cyclo (D)-proline-(L)-phenylalanine (10), cyclo (L)-proline-(L)-phenylalanine (11), 2-pyrrolidinone (12), N-methyl-2-pyrolidinone (13), cyclo-(L)-4-OH-proline-(L)-phenylalanine (14), brevianamide F (15), 3-methyl-piperazine-2,5-dione (16), 7,8-dimethylbenzo[g]pteridine-2,4 (1H,3H)-dione (17), cyclo (L)-tyrosine-(L)-phenylalanine (18), beer sterols (19), daidzein (20), and erythritol (21). Conclusion All compounds are isolated from Fusarium lactis for the first time.
[中图分类号]
[基金项目]
国家中医药管理局“中药化学”重点学科建设项目;安徽省高校自然科学基金重点项目(KJ2016A856)