[关键词]
[摘要]
目的 研究三七Panax notoginseng叶乙醇提取物的化学成分及抗氧化活性(ABTS和DPPH)。方法 利用硅藻土、硅胶、MCI柱色谱、凝胶柱色谱、中压制备液相色谱和制备液相等色谱技术进行分离纯化,并应用质谱、核磁共振等波谱技术鉴定化合物结构。初步考察了这些化合物的抗氧化能力。结果 从三七叶95%乙醇提取物的氯仿、丙酮-乙醇(4:1)和丙酮-乙醇(1:1)3个洗脱部位中分离鉴定了15个化合物,分别为falcarinol(1)、γ-tocopherol(2)、(5S)-3α-acetyl-2,3,5-trimethyl-7α-hydroxy-5-(4,8,12-trimethyltridecanyl)-1,3α,5,6,7,7α-hexahydro-4-oxainden-1-one(3)、picrionoside B(4)、linarionoside A(5)、viburnolide A(6)、槲皮素(7)、lilyn(8)、茵芋苷(9)、阿彼斯基姆素(10)、daphnin methylether(11)、20(S)-人参皂苷Rh2(12)、人参皂苷F2(13)、20(R)-人参皂苷Rh2(14)、异人参皂苷Rh3(15)。结论 化合物3、6、9 ~11 为首次从人参属植物中分离得到,化合物2、4 为首次从该植物中分离得到。化合物2、4、7、8 具有较强的抗氧化活性。
[Key word]
[Abstract]
Objective To study the chemical constituents from the leaves of Panax notoginseng and their antioxidant activity (ABTS and DPPH). Methods The constituents were isolated and purified by celite, silica gel, MCI and Sephadex LH-20, reversed-phase MPLC chromatographic methods, then purified by preparative HPLC. Their structures were identified on the basis of mass spectrum and NMR spectrum. Anti-oxidant activity of these compounds was initially investigated. Results Fifteen compounds were isolated from the chloroform, acetone-ethanol (4:1) and acetone-ethanol (1:1) fractions of 95% ethanol extract of the leaves of P. notoginseng, and their structures were elucidated as follows:falcarinol (1), γ-tocopherol (2), (2S,4αS,7αR)-7α-acetyl-3,4,4α,7α-tetrahydro-4α-hydroxy-2,6,7-trimethyl-2-(4,8,12-trimethyltridecyl) cyclopenta[β] pyran-5 (2H)-one (3), picrionoside B (4), linarionoside A (5), viburnolide A (6), quercetin (7), lilyn (8), skimmin (9), apiosylskimmin (10), daphnin methylether (11), 20 (S)-ginsenoside Rh2 (12), ginsenoside F2 (13), 20(R)-ginsenoside Rh2 (14), and isoginsenoside Rh3 (15). Conclusion Compounds 3, 6, and 9-11 are isolated from the plant of genus Panax for the first time, compounds 2 and 4 are isolated from this plant for the first time. Compounds 2, 4, 7, and 8 show certain anti-oxidant activity.
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[基金项目]
国家自然科学基金重点资助项目(81630094);中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-010)