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[摘要]
目的 研究人肠道菌群对汉黄芩苷的代谢作用。方法 采用UPLC-Q-TOF/MS技术分析肠道菌群对汉黄芩苷的代谢物;此外,采用16 S rDNA分子生物学方法进行肠道细菌的鉴定与组成分析。结果 汉黄芩苷主要被代谢为汉黄芩素和少量的脱甲基代谢物,且肠道混合菌群的代谢能力较强。多数Enterococcus属和Escherichia属的肠道细菌具有代谢功能,其中具有较强代谢活性的一种单菌为Escherichia sp.41。结论 汉黄芩苷主要被代谢为汉黄芩素和少量的脱甲基代谢物,鉴定出一种单菌为Escherichias sp.41。
[Key word]
[Abstract]
Objective To investigate the metabolic effects of wogonoside by human gut microbiota. Methods UPLC-Q-TOF/MS was used to analyze the metabolites of wogonoside. Additionally, 16 S rDNA molecular biology method was applied to the identification and composition analysis of intestinal bacteria. Results Wogonoside was mainly metabolized to wogonin and small quantities of demethylated metabolites. Most of Enterococcus and Escherchia had the metabolic abilities, among which a single bacterium Escherichia sp. 41 possessed the stronger metabolic activity. Conclusion This study has a good guiding significance for in vivo process and efficacy of wogonoside.
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[基金项目]
国家自然科学基金资助项目(81673831)