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[摘要]
目的 观察糖肾一号胶囊对糖尿病肾病(DN)大鼠的作用及可能机制。方法 SD大鼠采用ip链脲佐菌素(STZ)方法制备DN模型,造模成功大鼠随机分为模型组,贝那普利组(1.04 g/kg),糖肾一号胶囊高、中、低剂量(9、6、3 g/kg)组,每组15只,另设对照组15只,各组连续ig给药8周,测定各组大鼠血糖、血脂、血胱抑素C、血清固醇元件结合蛋白1c(SREBP-1c)、SREBP-2c水平,进行尿微量白蛋白定量,检测各组大鼠肾组织IRS-1、PI3K、podocin蛋白表达水平。结果 与模型组比较,糖肾一号胶囊高、中剂量组大鼠血糖、血脂、血胱抑素C、尿微量白蛋白明显降低(P<0.05、0.01),血清SREBP-1c和SREBP-2c水平显著降低(P<0.05),肾脏组织IRS-1、PI3K及podocin蛋白表达水平明显升高(P<0.05),而糖肾一号胶囊低剂量组升高不明显(P>0.05),高剂量组与中剂量组比较差异显著(P<0.05)。结论 糖肾一号胶囊对DN大鼠具有一定保护作用,其能够降低DN大鼠血糖、血脂、血胱抑素C、尿微量白蛋白水平,作用机制可能与提高肾组织podocin蛋白表达、改善肾足细胞损伤、延缓肾脏纤维化有关。
[Key word]
[Abstract]
Objective To observe effect and possible mechanism of Tangshen 1st Capsule on diabetic nephropathy rats. Methods Diabetic nephropathy model was established by SD rat ip injected with STZ. Dividing model rats randomly into six groups, 15 rats in model group, 15 rats in benazepril (1.04 g/kg) group, 15 rats in Tangshen 1st Capsule (9 g/kg), 15 rats in Tangshen 1st Capsule (6 g/kg), and 15 rats in Tangshen 1st Capsule (3 g/kg) group; In addition, there were 15 rats designated as normal control group. Rats in normal control group and model group were given corresponding drugs for 8 weeks. After 8 weeks, it was time to observe the blood glucose, blood lipid, cystain c, SREBP-1c, SREBP-2c, urinary albumin, expression of IRS-1, PI3K, and podocin protein levels in renal tissue. Results Compared with model group after treatment, in Tangshen 1st Capsule high-dose group and mid-dose group, blood glucose, blood lipid, cystain C, and urinary microalbumin decreased significantly (P<0.05, 0.01), SREBP-1c and SREBP-2c in serum decreased significantly (P<0.05); IRS-1, PI3K, and podocin protein expression in renal tissues increased significantly (P<0.05), and in low-dose group increased significantly (P>0.05), in high-dose group, and mid-dose group were statistically significant (P<0.05). Conclusion Tangshen 1st Capsule have protective effects on diabetic nephropathy rats, and its mechanisms are in part associated with reducing blood glucose and blood lipid, and increasing podocin protein expression.
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[基金项目]
陕西省教育厅科研项目(320104-203010017)