目的 研究薄荷Mentha haplocalyx在大鼠体内的入血成分及代谢产物。方法 采用超高效液相色谱-飞行时间质谱（UHPLC-Q-TOF-MS/MS）联用技术鉴定薄荷入血成分及代谢产物。采用Eclipse Plus C₁₈色谱柱（100 mm×4.6 mm，3.5 μm），流动相为0.1%甲酸水溶液（A）-乙腈（B），梯度洗脱，体积流量0.3 mL/min，进样量5 μL。结果 建立了大鼠给药后血浆样品的分析测定方法，并且与空白生物样品、薄荷提取物和对照品的保留时间及质谱离子碎片信息对比，共鉴定了28个入血原型成分、39个代谢产物，其中1个为未曾报道过的木犀草素的新代谢产物。结论 薄荷在大鼠体内的代谢途径包括氧化、还原、甲基化、硫酸酯化、葡萄糖醛酸化等，其中以Ⅱ相反应为主。所建立的方法简单可靠，为揭示薄荷的药效物质基础提供理论依据。

Objective To investigate the prototype components and metabolites of Mentha haplocalyx in rats plasma. Methods UHPLC-Q-TOF-MS/MS method was developed and applied to identify the components and metabolites of rat plasma. The analysis was carried out on an Eclipse Plus C₁₈ column (100 mm×4.6 mm, 3.5 μm, Agilent) with the mobile phase of 0.1% acetic acid solution (A)-acetonitrile (B) at a flowing rate of 0.3 mL/min, and the injection volume was 5μL. Results The developed method was applicable to the analysis and identification of metabolites of M. haplocalyx after oral administration. A total of 67 compounds, including 28 prototype components and 39 metabolites (one of which was a new metabolite of luteolin unreported), were identified by comparison of their retention time, ion fragmentation information with that of the blank biological samples, herb extract, and reference compounds. Conclusion The metabolic pathway of M. haplocalyx in rats were consisted of oxidation, reduction, methylation, sulfation, and glucuronidation, and the main metabolic pathway was phase Ⅱ metabolic pathway among of them. This experimental method is simple and reliable, which could provide theoretical basis for elucidating the bioactive components of M. haplocalyx.

江西省自然科学基金项目（20122BAB205038）；南昌大学研究生创新专项基金项目（cx2016295）