[关键词]
[摘要]
目的 研究金银花(忍冬Lonicera japonica干燥花蕾)的化学成分。方法 反复利用硅胶柱色谱、Sephadex LH-20、中压柱色谱及制备液相色谱等方法分离纯化;通过MS、1H-NMR及13C-NMR等技术鉴定化合物结构。结果 从金银花醋酸乙酯部位分离得到12个化合物,分别鉴定为对羟基苯甲酸(1)、香草酸(2)、槲皮素-3-O-α-L-吡喃鼠李糖苷(3)、山柰酚-3-O-β-D-吡喃葡萄糖苷(4)、3',4',7-三羟基-3,5-二甲氧基黄酮(5)、异鼠李素(6)、金丝桃苷(7)、槲皮素-7-O-β-D-吡喃葡萄糖苷(8)、环裂马钱子酸(9)、断氧马钱子苷半缩醛内酯(10)、5-O-咖啡酰奎宁酸甲酯(11)、豆甾醇(12)。结论 化合物5首次从忍冬属植物中分离得到;化合物1~4、6、11、12首次从金银花中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents of the flower buds of Lonicera japonica. Methods The chemical constituents were isolated by repeated silica gel chromatography, Sephadex LH-20, medium pressure column chromatography and preparative HPLC, and their structures were elucidated by spectroscopic analyses and comparison of MS, 1H-NMR and 13C-NMR data with those reported in literature. Results Twelve compounds from the EtOAc fraction of L. japonica included p-hydroxybenzoic acid (1), vanillic acid (2), quercetin-3-O-α-L-rhamnopyranoside (3), kaemnpferol-3-O-β-D-glucopyranoside (4), 3',4',7-trihydroxy-3,5-dimethoxyflavone (5), isorhamnetin (6), hyperoside (7), quercetin-7-O-β-D-glucopyranoside (8), secologanic acid (9), vogeloside (10), 5-O-caffeoylquinic acidmethylester (11) and stigmasterol (12). Conclusion Compound 5 is obtained from the plant in Lonicera Linn. for the first time; Compounds 1-4, 6, 11 and 12 are obtained from this plants for the first time.
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[基金项目]
科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)