[关键词]
[摘要]
目的 研究丹参不同提取物的超高效液相色谱(UPLC)图谱与抗炎作用的谱效关系,为明确丹参抗炎作用物质基础提供依据。方法 采用UPLC法对丹参不同提取部位(S1~S9)进行分析,以人支气管上皮细胞炎症模型考察不同提取部位的抗炎作用,采用灰色关联度分析法建立其谱效关系。结果 丹参醇提物50%乙醇洗脱液(S3)、80%乙醇洗脱液(S4)、滤过沉淀(S5)以及萃取后水层提取物(S9)对于炎症细胞抗炎能力强,其中以丹参醇提物的50%乙醇洗脱液(S3)作用能力最强。丹参发挥抗炎作用的药效是多种成分共同作用的结果,各特征峰所代表的化学成分对其抗炎药效贡献的大小顺序为9号峰 > 1号峰 > 2号峰 > 6号峰 > 15号峰 > 11号峰 > 12号峰 > 7号峰 > 10号峰 > 5号峰 > 14号峰 > 3号峰 > 18号峰 > 4号峰 > 13号峰 > 16号峰 > 8号峰,对抗炎作用药效贡献大的前4个峰分别为9、1、2、6号峰,经过与对照品比对及质谱分析鉴定出4个峰对应的成分分别为丹参酮IIA、丹参素、丹酚酸B和3'-甲基丹酚酸B。结论 丹参抗炎作用的药效是多种成分共同作用的结果,明确了丹参中丹参酮IIA、丹参素、丹酚酸B和3'-甲基丹酚酸B这4个成分对其抗炎作用的药效贡献最大,为今后探索丹参药材抗炎作用的药效物质基础提供了一种新思路。
[Key word]
[Abstract]
Objective To study the relationship between UPLC and anti-inflammatory effects of different extracts of Salvia miltiorrhiza, and to provide a basis for clarifying the material basis of anti-inflammatory effects of S. miltiorrhiza. Methods UPLC was used to analyze the different extraction sites of S. miltiorrhiza. The anti-inflammatory effect of anti-bronchial epithelial cell inflammation model was studied by different extraction sites. The spectral relationship was established by gravitational correlation analysis (GRA). Results 50% grams of ethanol extract (S3), 80% ethanol eluent (S4), filtered precipitate (S5), and extracted aqueous layer extract (S9) had strong anti-inflammatory ability to inflammatory cells. The 50% ethanol eluate (S3) of the ethanol extract of Danshen was the strongest. The effect of chemical composition on the anti-inflammatory efficacy of each characteristic peak was as the following order:A9 > A1 > A2 > A6 > A15 > A11 > A12 > A7 > A10 > A5 > A14 > A3 > A18 > A4 > A13 > A16 > A8, and the top four peaks with strong contribution to anti-inflammatory effects were peaks 9, 1, 2, and 6, respectively. The results were as follows:tanshinone ⅡA, danshensu, salvianolic acid B, and 3'-methyl salvianolic acid B. Conclusion The anti-inflammatory efficacy of S. miltiorrhiza is the result of combination of various components. It is clear that the four components are tanshinone ⅡA, danshensu, salvianolic acid B, and 3'-methyl salvianolic acid B. The efficacy of the largest contribution to the future to explore the efficacy of anti-inflammatory effects of S. miltiorrhiza provides new ideas.
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[基金项目]
国家科技支撑计划项目(2011BAI07B08)