目的 研究含白芍中成药逍遥丸对血虚肝郁证大鼠外周血象和下丘脑-垂体-肾上腺轴的影响。方法 以辐照结合慢性束缚应激的方法制备大鼠血虚肝郁模型。健康雄性SD大鼠随机分为对照组、模型组、白芍组、逍遥丸组，对照组常规饲养，其余各组单笼饲养。造模第1天开始ig给药，共给药21 d。检测大鼠体质量、外周血象、糖水消耗量，并于给药结束后测定血浆中促肾上腺皮质激素（ACTH）、皮质酮（CORT）、下丘脑促肾上腺皮质激素释放素（CRH）的量。结果 与对照组相比，模型组大鼠体质量、外周血象白细胞（WBC）量明显降低（P<0.001），ACTH、CORT、CRH量均明显升高（P<0.01、0.001），糖水消耗实验表明模型组大鼠快感缺失（P<0.001），逍遥丸对上述各项指标具有相应的调节作用（P<0.01、0.001）。结论 逍遥丸对外周血象及HPA轴的调整作用可能是其发挥疏肝解郁、养血健脾功效的机制之一。

Objective To study the effect of Xiaoyao Pills (containing Paeoniae Alba Radix) on peripheral blood and hypothalamic-pituitary-adrenocortical (HPA) axis in rats with syndrome of stagnation of liver qi and blood deficiency. Methods The model of syndrome of stagnation of liver qi and blood deficiency was established by chronic restraint stress combined with radiation, and healthy male rats were randomly divided into four groups such as normal group, model group, Paeoniae Alba Radix group, and Xiaoyao Pills group. The rats in treatment groups and normal group were fed with medicines and pure water from day 1 of the experiment, respectively. The weight, peripheral blood, glucose consumption, and hypothalamic-pituitary-adrenal axis were measured. Results Compared with the model group, the weight and amount of WBC of model group were decreased significantly (P<0.001), and the amounts of ACTH, CORT, and CRH were increased significantly (P<0.01 or 0.001). Glucose consumption test showed that the ability of model group to explore the novel environment was reduced (P<0.01), Xiaoyao Pills group on the above indicators had a corresponding regulatory role (P<0.001 or P<0.01). Conclusion The effect of Xiaoyao Pill on peripheral blood and HPA axis may be one of the mechanisms of its function of soothing liver to resolve depression and nourishing blood to invigorate the spleen.

国家自然科学基金资助项目（81473370）