[关键词]
[摘要]
目的 比较参术益肠丸细粉和超微粉中主要活性成分葛根素、芍药苷和甘草苷溶出情况,为其生产工艺优化提供实验依据。方法 采用高效液相色谱法分别对参术益肠丸中主要活性成分在不同溶出介质中相对累积溶出率进行定量研究。色谱条件:Agilent Eclipse XDB-C18色谱柱(150 mm×4.6 mm,5 μm);流动相为乙腈-0.1%磷酸水溶液(11:89);体积流量1.5 mL/min;检测波长230 nm;进样量10 μL。结果 细粉中葛根素和甘草苷在水和人工胃液中相对累积溶出率大于超微粉,而细粉中芍药苷在水和人工胃液中相对累积溶出率小于超微粉。人工肠液中超微粉和细粉的溶出行为相似。结论 适度的微粉化能促进参术益肠丸有效成分的溶出,其溶出行为还受到不同溶出介质的影响,超微粉碎技术应用于参术益肠丸还需进一步研究。
[Key word]
[Abstract]
Objective To compare the differences in the dissolution behavior of puerarin, paeoniflorin, and liquiritin between ultrafine powder and fine powder of Shenzhu Yichang Pills to provide experimental evidence for optimize the production technology. Methods The relative cumulative dissolution rates of powders of Shenzhu Yichang Pills in different dissolution mediums were quantitatively studied by HPLC column of Agilent Eclipse XDB-C18 (150 mm×4.6 mm, 5 μm), mobile phase of CH3CN-0.1% H3PO4 (11:89), flow rate of 1.5 mL/min, detection wavelength of 230 nm, and the volume of injection was 10 μL. Results The relative cumulative dissolution rates of puerarin and liquiritin in distilled water and artificial gastric fluid for fine powder were bigger than that for ultrafine powder, but the relative cumulative dissolution rates of paeoniflorin in distilled water and artificial gastric fluid for fine powder were smaller than that for ultrafine powder. The dissolution behavior of fine powder was similar to that of ultrafine powder in artificial intestinal fluid. Conclusion An appropriate degree of ultrafine grinding can promote the dissolution of active ingredients of Shenzhu Yichang Pills, and the dissolution is influenced by the dissolution medium. Further research is needed to the application of ultrafine grinding technology for Shenzhu Yichang Pills.
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[基金项目]
芜湖市科技惠民项目(2014hm31);安徽省中医药科研课题(2016zy41)