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[摘要]
目的 建立金水宝胶囊发酵虫草菌粉多糖的指纹图谱。方法 沸水回流提取金水宝胶囊中的发酵虫草菌粉多糖,经酸水解和1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化后采用HPLC法分析,色谱条件为Phenomenex OOG-4252-EO C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.05 mol/L磷酸盐缓冲液梯度洗脱,体积流量1 mL/min,进样量20 μL,检测波长250 nm,柱温30℃。结果 对组成发酵虫草菌粉多糖的11种单糖成分进行鉴别,并建立了发酵虫草菌粉多糖指纹图谱。通过指纹图谱分析10批金水宝胶囊中各单糖成分,相似度均大于0.999,无显著性差异。结论 建立的发酵虫草菌粉多糖指纹图谱操作简单,专属性强,重复性好,能够客观评价金水宝胶囊的质量。
[Key word]
[Abstract]
Objective To establish a polysaccharide fingerprint analysis method for identifying Jinshuibao Capsule. Methods Polysaccharide from fermentation Cordyceps powder in Jinshuibao Capsule was extracted by boiling water, hydrolyzed by acid, and precolumn derivatization method for 1-phenyl-3-meth yl-5-pyrazolone (PMP), analyzed by HPLC. The chromatographic conditions were Phenomenex OOG-4252-EO C18 column (250 mm×4.6 mm, 5 μm), eluting with acetonitrile-0.05 mol/L phosphate buffer salt gradient, and the detection wavelength was 250 nm. The column temperature was 30℃. Results Eleven monosaccharide components that made up the fermentation Cordyceps powder were identified and established the fingerprint of fermentation Cordyceps powder polysaccharide. Ten batches of monosaccharide components in Jinshuibao Capsule were analyzed by fingerprints. The similarity was greater than 0.99 with no significant difference. Conclusion The fingerprints of fermentation Cordyceps powder were simple, specific and reproducible, and the quality of Jinshuibao Capsule is evaluated objectively.
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[基金项目]
国家中药标准化项目(ZYBZH-C-JX-39);国家“重大新药创制”科技重大专项(2011ZX09201-201-30);江西省高等学校科技落地计划项目(产学研合作KJLD13061)