目的 对冬凌草内生真菌进行筛选鉴定，并研究其体外抗肿瘤活性，以期得到冬凌草甲素高产抗肿瘤活性菌株。方法 采用TLC法和HPLC法对前期从冬凌草中分离出的256株内生真菌进行筛选，HPLC-MS测定菌株发酵液中的冬凌草甲素。结合形态学特征、ITS序列对筛选出的菌株进行鉴定。MTT法测定筛选菌株的体外抗肿瘤活性。结果 从冬凌草中分离出的256株内生真菌经筛选，获得1株冬凌草甲素高产菌株M-J-5，鉴定为青霉属草酸青霉菌Penicillium oxalicum Currie et Thom，其对人乳腺癌细胞系MCF-7细胞活性具有一定抑制作用。结论 从冬凌草内生真菌中筛选得到产冬凌草甲素且具抗肿瘤活性的菌株M-J-5。

Objective To select and identify the endophytic fungi of Rabdosia rubescens as well as study on its antitumor activity in vitro in order to obtain the high yield strains of oridonin. Methods Using the methods of TLC and HPLC, the oridonin high yield strain was selected from 256 strains of endophytic fungi which were isolated from the previous experiments. Oridonin of fermentation broth was determined by HPLC-MS. The strain was identified by morphological characteristics and ITS sequence methods and its antitumor activity in vitro was detected by MTT assay. Results An oridonin high yield strain M-J-5 was obtained and identified as Penicillium oxalicum, which can inhibit the activity of human breast cancer cell line MCF-7. Conclusion a oridonin high yield strain is obtained with antitumor activity and provides scientific basis for microbial production of oridonin and the development of new anticancer drugs.

国家自然科学基金资助项目（81603232）