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[摘要]
目的 对浙江七叶树Aesculus chinensis var. chekiangensis种子的70%乙醇提取物进行化学成分研究。方法 采用D101大孔吸附树脂柱色谱、正相硅胶柱色谱、Sephadex LH-20柱色谱、反相高效液相色谱等方法对浙江七叶树种子的化学成分进行分离纯化,并根据理化性质和波谱数据等鉴定其结构。结果 从浙江七叶树种子中共分离得到10个化合物,分别鉴定为2R,3R-3,7,3'-三羟基-5'-甲氧基二氢黄烷-5-O-β-D-葡萄糖苷(1)、槲皮素-4'-O-β-D-葡萄糖苷(2)、槲皮素-3'-O-β-D-葡萄糖苷(3)、槲皮素-3-O-[β-D-木糖基(1-2)]-β-D-葡萄糖苷(4)、槲皮素-3-O-[β-D-吡喃葡萄糖(1-4)]-α-L-吡喃鼠李糖苷(5)、山柰酚-3-O-[β-D-吡喃葡萄糖(1-4)]-α-L-吡喃鼠李糖苷(6)、山柰酚-3-O-[β-D-木糖基(1-2)]-β-D-葡萄糖苷(7)、山柰酚-3-O-[β-D-木糖基(1-2)][β-D-葡萄糖基(1-3)]-β-D-葡萄糖苷(8)、(-)-表儿茶素(9)、槲皮素(10)。结论 化合物1、2为首次从七叶树属植物中分离得到,化合物3~9为首次从浙江七叶树种子中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents from the 70% ethanolic extracts of Aesculus chinensis seeds. Methods The compounds were isolated by D101 macroporous adsorptive resins, silica gel, Sephadex LH-20, and preparative-HPLC and their structures were identified by chemical methods and spectroscopic analyses. Results Ten compounds were isolated from the 70% ethanolic extracts of A. chinensis seeds. They were 2R,3R-3,7,3'-trihydroxy-5'-methoxyflavane-5-O-β-glucopyranoside (1), quercetin-4'-O-β-D-glucopyranoside (2), quercetin-3'-O-β-D-glucoside (3), quercetin-3-O-[β-D-xylopyranosyl (1-2)]-β-D-glucopyranoside (4), quercetin-3-O-[β-D-glucopyranosyl (1-4)]-α-L-rhamnopyranoside (5), kaempferol-3-O-[β-D-glucopyranosyl (1-4)]-α-L-rhamnopyranoside (6), kaempferol-3-O-[β-D-xylopyranosyl (1-2)]-β-D-glucopyranoside (7), kaemferol-3-O-[β-D-xylopyranosyl (1-2)] [β-D-glucopyranosyl (1-3)]-β-D-glucopyranoside (8), (-)-epicatechin (9), and quercetin (10). Conclusion Compounds 1 and 2 are obtained from genus Aesculus Linn. for the first time, and compounds 3—9 are isolated from A. chinensis for the first time.
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[基金项目]
国家自然科学基金重点项目(81430095)