目的 鉴定大鼠口服桑叶黄酮提取物后血浆、尿液、粪便、胆汁样品中的化学物质。方法 Wistar大鼠按0.5 g/kg一次性ig桑叶黄酮提取物后，取不同时间点的血浆、尿液、粪便及胆汁，采用超高效液相色谱与四级杆飞行时间质谱联用（UPLC-Q-TOF/MS）技术对各样品进行检测，分析样品中存在的化学物质。结果 从大鼠血浆、尿液、粪便、胆汁中共鉴定出13个原型成分，41个代谢产物，其代谢产物以葡萄糖醛酸结合、硫酸结合及甲基化产物为主。结论 该研究为桑叶的药动学及药效作用机制研究提供一定的参考。

Objective To identify the chemical substances in plasma, urine, feces and bile samples of rats after oral administration of mulberry leaves flavonoids. Methods The plasma, urine, feces and bile samples of rats after a single oral administration of mulberry leaves flavonoids at a dose of 0.5 g/kg at different time points were detected by UPLC-Q-TOF/MS. Results Thirteen prototype compounds and 41 metabolites were found. The metabolites mainly included glucuronidation, sulfation and methylation. Conclusion This reseach estabilished the basis to study the pharmacokinetics and the pharmacological action mechanism of the mulberry leaves.

国家自然科学基金重点资助项目（81430095）；“重大新药创制”科技重大专项（2012ZX09101212）