[关键词]
[摘要]
目的 建立桃儿七的HPLC指纹图谱分析方法,为该药材的鉴别及质量评价提供依据。方法 采用Thermo HyPURITY C18色谱柱(250 mm×4.6 mm,5 μm),以甲醇-0.4%磷酸水溶液为流动相,进行梯度洗脱,柱温25 ℃,体积流量0.8 mL/min,检测波长290 nm,进样量10 μL。采用“中药色谱指纹图谱相似度评价系统”(2004 A)和SPSS 19.0统计软件中的聚类分析对指纹图谱进行相似度分析。结果 建立桃儿七的HPLC指纹图谱,确定了17个共有峰,并指认了其中8个共有峰,经相似度评价,19批桃儿七药材指纹图谱与共有模式比较的相似度均在0.9以上,整体相似度较好,聚类结果与相似度评价结果基本一致。结论 该研究建立的桃儿七HPLC指纹图谱可为该药材的鉴别及质量评价提供更全面的参考。
[Key word]
[Abstract]
Objective The HPLC fingerprint of Sinopodophylli Radix et Rhzoma was established, which provided the basis for the identification and quality evaluation of the medicinal materials. Methods The chromatographic separation was performed on a Thermo HyPURITY C18 column (250 mm × 4.6 mm, 5 μm) using methanol (A)-0.4% orthophosphoric acid (B) as mobile phase with gradient elution. The column temperature was 25 ℃, the flow rate was 0.8 mL/min, the detection wavelength was set at 290 nm, and the injection volume was 10 μL. The fingerprints were compared for similarity using “Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System” (2004 A) issued by Chinese Pharmacopoeia commission and cluster analysis in SPSS 19.0. Results HPLC fingerprint of Sinopodophylli Radix et Rhzoma had 17 common peaks, and eight of them were identified. In the similarity evaluation, The HPLC fingerprint of 19 batches of Sinopodophylli Radix et Rhzoma had a similarity ratio of more than 0.9 when compared with the mutual mode HPLC fingerprints. The overall similarity was good, and the cluster analysis result was basically the same as the similarity evaluation result. Conclusion The HPLC fingerprint of Sinopodophylli Radix et Rhzoma can provide more comprehensive reference for its identification and quality evaluation.
[中图分类号]
[基金项目]
陕西省中药基础与新药研究重点实验室资助;陕西省科技统筹创新计划项目(2013KTCQ03-14)