目的 采用液相-质谱联用（LC-MS/MS）分析硫磺熏蒸（硫熏）对浙贝母中贝母素甲、贝母素乙在大鼠体内药动学的影响。方法 SD大鼠分别ig给予硫熏、鲜切浙贝母提取物（生药剂量15 g/kg），采用LC-MS/MS技术检测贝母素甲和贝母素乙的血药浓度，并用3P97软件计算药动学参数。结果 硫熏浙贝母中贝母素甲和贝母素乙的主要药动学参数：达峰浓度（C_max）分别为（66.40±4.65）、（146.72±10.88）ng/mL，药时曲线下面积（AUC_0~t）分别为（181.79±7.85）、（457.38±58.81）ng∙h/mL；鲜切浙贝母中贝母素甲和贝母素乙的主要药动学参数：C_max分别为（186.37±18.80）、（227.65±7.01）ng/mL，AUC_0~t分别为（197.70±18.69）、（566.16±41.55）ng∙h/mL。硫熏浙贝母组大鼠体内贝母素甲、贝母素乙的C_max、AUC_0~t明显低于鲜切浙贝母组。结论 硫熏降低浙贝母中贝母素甲、贝母素乙的血药浓度，影响浙贝母中主要生物碱贝母甲素和贝母乙素的药动学行为。

Objective To study the influence of sulfur-fumigation on pharmacokinetic of peimine and peiminine of Fritillaria thunbergii in rat plasma by LC-MS/MS. Methods After random grouping, 18 SD rats were given the solution of fresh-cut and sulfur-fumigated F. thunbergii by ig administration. The blood drug concentration of peimine and peiminine in rat plasma was determined by HPLC-MS/MS, and the pharmacokinetic parameters were calculated with 3P97 software. Results The pharmacokinetic parameters of peimine and peiminine of sulfur-fumigated F. thunbergii in rat plasma were (66.40 ± 4.65), (146.72 ± 10.88) ng/mL for C_max, and (181.79 ± 7.85), (457.38 ± 58.81) ng∙h/mL for AUC, respectively. Those of fresh-cut sample in rat plasma were (186.37 ± 18.8), (227.65 ± 7.01) ng/mL for C_max, and (197.70 ± 18.69), (566.16 ± 41.55) ng∙h/mL for AUC, respectively. The pharmacokinetic parameters of perimine and peiminine of sulfur-fumigated sample in rat plasma were less than those of fresh-cut sample. Conclusion The results showed that sulfur-fumigation decreased the bioavailability of peimine and peiminine. This study could provide a basis for further clarifying the influence of sulfur-fumigation on efficacy material base of F. thunbergii.

国家中药标准化项目（ZYBZH-Y-ZY-45）；国家中医药行业专项（201507002）；浙江省公益性技术应用研究计划（2015C33152）；浙江省中医药科学研究基金计划（2012ZB032，2013ZA027）；浙江中医药大学重中之重一级学科——中药学学科开放基金