目的 一测多评（QAMS）法测定畲药食凉茶中4种黄酮类成分，并以其研究食凉茶黄酮类物质的动态变化规律。方法 以芦丁为参照物，分别建立其与山柰酚-3-O-芸香糖苷、槲皮素、山柰素的相对校正因子（f），并计算食凉茶中各成分量，实现QAMS。同时采用外标法验证QAMS法的准确性和可行性。结果 芦丁与山柰酚-3-O-芸香糖苷、槲皮素和山柰素的f分别为1.158、0.475 6、0.431 9，RSD分别为0.73%、1.55%、1.58%；QAMS法测定结果与外标法测定结果无显著差异。不同月份食凉茶中黄酮类成分质量分数差异明显，2个来源食凉茶之间的量差异亦较明显。结论 以芦丁为参照物同时测定山柰酚-3-O-芸香糖苷、槲皮素、山柰素的QAMS法可用于畲药食凉茶的定量分析。以黄酮类物质为指标，食凉茶的最佳采收期为7月和8月。

Objective To establish an assay method of flavonoids (four components) in traditional SHE medicine-Shiliang Tea by quantitative analysis of multi-components by single-marker (QAMS), and to analyze the dynamic change of flavonoids at different harvest time and different collection places. Methods With rutin as the internal reference substance, the relative correction factor (RCF) of kaempferol-3-O-rutinoside, quercetin, and kaempferol was calculated. Then the contents of four components were calculated, and the accuracy and feasibility of method was evaluated through external standard method. Results The RCF of rutin to kaempferol-3-O-rutinoside, quercetin, and kaempferol were 1.158 with RSD 0.73%, 0.475 6 with RSD 1.55%, 0.431 9 with RSD 1.58%, respectively. There was no significant difference of assay results between QAMS method and external standard method. While the differences of content between different harvest months and two different species were significant. Conclusion The QAMS method with rutin as internal reference substance can be used for quantitative analysis of four flavonoids in Shiliang Tea. It is suggested that the best harvest time of Shiliang Tea for flavonoids is in July and August.

丽水市高层次人才培养项目（2015RC25）；浙江省食品药品监督管理与产业发展研究会项目（ZYH2016013）