[关键词]
[摘要]
目的 探究大黄Rheum officinale提取物(ROE)对果蝇肠道干细胞(ISC)活性的影响。方法 对照组果蝇食用正常玉米粉-酵母培养基,实验组果蝇食用含有0.05 g/mL或0.1 g/mL ROE的玉米粉-酵母培养基。对果蝇喂食有毒化合物诱导肠道损伤。分别检测分析果蝇的生存率、肠道前体细胞形态数目、ISC的增殖与分化、活性氧自由基(ROS)水平、肠上皮死亡细胞数目以及果蝇的寿命。结果 0.05 g/mL和0.1 g/mL ROE均可以提高肠道损伤后果蝇的生存率,0.1 g/mL ROE可以降低十二烷基硫酸钠(SDS)诱导后ROS的水平,减少死亡细胞的数目,抑制ISC的过度增殖与分化,缓解前体细胞的过度积累,维持肠道内稳态。此外ROE还可以延长果蝇寿命。结论 ROE可以抑制ISC的过度增殖和分化,增强果蝇肠道免疫功能,延长果蝇寿命。
[Key word]
[Abstract]
Objective To explore the effect of Rheum officinale extracts (ROE) on activity of intestinal stem cells of Drosophila melanogaster. Methods The control group was fed with normal cornmeal-yeast medium, and the experimental group was fed with cornmeal-yeast medium containing 0.05 or 0.1 g/mL ROE. In the experiment, the gut damage was induced by feeding .D melanogaster with toxic compounds. The effects of ROE on survival rate, number and morphology of progenitor cells, proliferation and differentiation of intestinal stem cells, expression of reactive oxygen species (ROS), number of intestinal epithelial dead cells and life span of .D melanogaster were detected and analyzed. Results ROE (0.05 and 0.1 g/mL) could increase the survival rate of .D melanogaster induced by toxic compounds. ROE (0.1 g/mL) could decrease SDS-induced ROS levels, reduce the number of intestinal epithelial dead cells, inhibit excessive proliferation and differentiation of intestinal stem cell, alleviate the excessive accumulation of progenitor cells, thereby maintain homeostasis in the gut. In addition, ROE could prolong the lifespan of .D melanogaster. Conclusion ROE can inhibit excessive proliferation and differentiation of intestinal stem cells, enhance gut immune function, and prolong the life span of .D melanogaster.
[中图分类号]
[基金项目]
中央高校基本科研业务费专项资金(2572016EAJ4)