目的 建立高乌头炮制前后镇痛抗炎活性部位指纹图谱，从物质基础上揭示高乌头炮制后镇痛抗炎活性增强的原因。方法 通过HPLC梯度洗脱分别建立10批不同地区高乌头药材炮制前后三氯甲烷萃取部分的指纹图谱，并采用中药指纹图谱相似度评价系统（2012版）进行分析。结果 建立了生、制高乌头三氯甲烷活性部位HPLC指纹图谱；在生、制品三氯甲烷活性部位图谱中分别标出3个共有峰、15个共有峰，制品新增12个峰，其中1、2、7号峰增加特别明显，占新增峰面积的72.3%~84.5%；同时对生、制品三氯甲烷部分2个共有成分（高乌甲素、冉乌头碱）进行定量测定，炮制后其量均降低，冉乌头碱量降低至原来的1/3。结论 该方法重现性好、特征性强，可用于高乌头生、制品镇痛抗炎活性部位的质量评价，为阐明高乌头炮制前后物质基础变化及炮制原理提供科学依据。

Objective To set up an analysis method of fingerprints for analgesic and anti-inflammatory effective parts from crude and processed roots of Aconitum sinomontanum (AS), and to discuss the chemical composition changes after processing that enhance the analgesic and anti-inflammatory effect. Methods HPLC gradient elution method was developed to establish fingerprints for 10 batches of chloroform extract from crude and processed the roots of AS in different areas. And the fingerprint were analyzed and compared by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2012 edition). Results The fingerprints of chloroform extract from crude and processed the roots of AS were set up by HPLC. The gained 3 and 15 common peaks from crude and processed roots of AS, respectively. processed product added 12 peaks, including 1 peak, 2 peak, 7 peak increase were significant, accounting for the new peak area of 72.3%-84.5%. And determination of lappacontine and ranaconitine of chloroform extract from crude and proceed products, after processing the content were reduced, ranaconitine content reduced to the original one-third. Conclusion This method with good reproducibility, and strong characteristic, and could be used for the full quality evaluation of analgesic and anti-inflammatory effect parts from crude and processed the roots of AS. To provide scientific basis for elucidating the chemical substance base and processing principle of crude and processed roots of AS.

国家自然科学基金项目（81560650）；甘肃省自然科学基金项目（1107RJZA242）