目的 研究大鼠ig毛蕊异黄酮-7-O-β-D-葡萄糖苷后尿中代谢产物。方法 采用制备液相进行分离和纯化，通过核磁和质谱数据确定化合物的结构。结果 从大鼠ig毛蕊异黄酮-7-O-β-D-葡萄糖苷后的尿中提取分离得到5个代谢产物，分别为毛蕊异黄酮（M₁）、3',4',7-三羟基异黄酮（M₂）、大豆素（M₃）、毛蕊异黄酮-3'-O-β-D-葡萄糖醛酸苷（M₄）、毛蕊异黄酮-3'-O-β-D-葡萄糖醛酸甲酯苷（M₅）。结论 代谢产物M₅为新的化合物。

Objective To study the metabolites from rats urine after ig administration of calycosin-7-O-β-D-glucopyranoside. Methods The constituents were isolated and purified by preparative liquid chromatographic technique, and the structures were identified by spectroscopic analyses including ESI-MS, NMR, and 2D-NMR. Results Five metabolites were isolated from rats urine after ig administration of calycosin-7-O-β-D-glucopyranoside. They were identified as calycosin (M₁), 3',4',7-trihydroxyisoflavone (M₂), daidzein (M₃), calycosin-3'-O-β-D-glucuronide (M₄), and calycosin-3'-O-β-D-glucuronide methyl ester (M₅). Conclusion M₅ is a new compound.