[关键词]
[摘要]
目的 通过对不同产地荆芥Schizonepeta tenuifolia中6种黄酮类成分的检测,为其质量控制提供依据。方法 采用高效液相色谱(HPLC)法建立荆芥药材中黄酮类成分的检测方法,并以6种黄酮类成分的量为数据来源,对其进行聚类分析。结果 不同产地荆芥中6种黄酮类成分的量差异较大,木犀草苷、槲皮苷、橙皮苷、木犀草素、芹菜素、香叶木素的线性范围分别是0.030~0.148 μg、0.222~1.108 μg、0.357~1.784 μg、0.058~0.292 μg、0.054~0.269 μg、0.050~0.247 μg,加样回收率为96.32%~99.97%,RSD<2.20%;其中,安徽产地聚为一类,河南1与广东产地为聚一类,河南2、河北、云南、四川、湖北、甘肃产地聚为一类。结论 建立的方法简单、灵敏,可用于荆芥的多指标成分的质量控制;其次,河南1、河南2、河北、云南、四川、湖北、广东、甘肃的亲缘关系较近,可以作为同一药用来源。
[Key word]
[Abstract]
Objective To detect the contents of six kinds of flavonoids in Schizonepeta tenuifolia from different areas, so as to provide a basis for their quality control. Methods Ultra performance liquid chromatography (UPLC) was used to establish the detection scheme of flavonoids content, and clustering analysis was conducted by the results of content detection. Results The contents of six kinds of flavonoids in S. tenuifolia from different areas had large differences. The linear ranges were:0.030-0.148 μg for cynaroside, 0.222-1.108 μg for quercetin, 0.357-1.784 μg for hesperidin, 0.058-0.292 μg for luteolin, 0.054-0.269 μg for apigenin, and 0.050-0.247 μg for diosmetin, respectively. The average recovery rate was between 96.32% and 99.97%, RSD < 2.20%; Among this, the Schizonepeta Briq. herb from Anhui was as one class, the herbs from Henan 1 and Guangdong were as one class, and the herbs from Henan 2, Hebei, Yunnan, Sichuan, Hubei, and Gansu were as the other class. Conclusion The method used for quality control of the multi-index level in this experiment is simple and sensitive; on the other hand, the herbs from Henan 1, Henan 2, Hebei, Yunnan, Sichuan, Hubei, Guangdong and Gansu have a close relationship; They can be used as the same medicinal source.
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[基金项目]
“十二五”重大新药创制科技重大专项(2013ZX09507005-005);辽宁省教育厅一般项目(L201605,L201608)