[关键词]
[摘要]
目的 研究壮药两粤黄檀Dalbergia benthami的化学成分。方法 通过硅胶、凝胶、HPLC等柱色谱及重结晶方法进行分离和纯化,根据理化性质和波谱数据对化合物结构进行鉴定。结果 从两粤黄檀乙醇提取物中分离得到14个化合物,分别鉴定为4'-羟基-6-羟甲基-5,7-二甲氧基异黄酮(1)、β-谷甾醇(2)、胡萝卜苷(3)、豆甾醇(4)、大鱼藤树酸(5)、robustin(6)、4-hydroxy-3-(3'-hydroxy-4'-methoxyphenyl)-5-methoxy-2",2"-dimethylpyrano-(5",6":6,7)-coumarin(7)、大鱼藤树素甲醚(8)、大鱼藤树酸甲酯(9)、4'-羟基-5,7-二甲氧基-6-(3-甲基-2-丁烯基)-异黄酮(10)、芒柄花黄素(11)、伪赝靛苷元(12)、毛蕊异黄酮(13)、豆甾醇-3-O-β-D-葡萄糖苷(14)。结论 化合物1为新化合物,命名为两粤黄檀酮;所有化合物均为首次从该属植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents of Zhuang medicine Dalbergia benthami. Methods The compounds were isolated and purified by silica gel, Sephadex LH-20 chromatography, HPLC and so on. The structures of the compounds were identified on the basis of chemical and spectral methods. Results Fourteen compounds were isolated from the ethanol extract of Dalbergia benthami and identified as 4'-hydroxy-6-hydroxymethyl-5,7-dimethoxy isoflavone (1), β-sitosterol (2), daucosterol (3), stigmasterol (4), robustin acid (5), robustin (6), 4-hydroxy-3-(3'-hydroxy-4'-methoxyphyl)-5-methoxy-2",2"-dimethylpyrano (5",6":6,7)coumarin (7), robustin methyl ether (8), methyl robustate (9), 4'-hydroxy-5,7-dimethoxy-6-(3-methyl-2-butenyl)-isoflavone (10), formononetin (11), pseudobaptigenin (12), calycosin (13), and stigmasterol-3-O-β-D-glucopyranoside (14). Conclusion Compound 1 is a new compound named dalbergibenthamone, and all compounds are isolated from this plant for the first time.
[中图分类号]
[基金项目]
广西自然科学基金项目(2014GXNSFAA118213);广西高校科学技术研究项目(ZD2014071);2015年广西优势特色重点学科——中药学(J15002);广西卫生厅中医药民族医药继承创新工程课题(GZZC 13-01,GZZC13-02);广西高校中药提取纯化与质量分析重点实验室(桂教科研〔2014〕6号)