[关键词]
[摘要]
目的 研究新疆荒地阿魏Ferula syreitschikowii 种质资源的亲缘关系及遗传多样性。方法 以96份新疆荒地阿魏种质资源为材料,从33条ISSR引物中筛选出条带清晰、多态性好的引物15条,进行ISSR分子标记分析。结果 共扩增获得292条完整、清晰的谱带,其中多态性条带为289条,多态位点比率为99.01%,表明新疆荒地阿魏种质资源多态性较高,具有较高的遗传多样性。经相关统计软件计算结果分析,平均有效等位基因数为1.725 6,平均Nei's基因多样性指数为0.404 8,平均Shannon's信息指数为0.587 9,遗传相似性系数为0.500 0~0.828 7。UPGMA聚类结果显示96份材料分为2个类群13个亚类,能够将不同来源的种质资源区分开来。结论 从分子水平上揭示了新疆荒地阿魏地理分布与种质资源间的亲缘关系及较高的遗传多样性,为荒地阿魏品种鉴定提供相关依据。
[Key word]
[Abstract]
ObjectIve To study the genetic relationship and genetic diversity of Ferula syreitschikowii germplasm resources in Xinjiang province. Methods A total of 96 F. syreitschikowii germplasm resources of Xinjiang province were used as experimental materials. Fifteen primers selected from 33 ISSR primers could obtain high polymorphism and reproducibility bands for ISSR molecular marker analysis. Results Total by 292 DNA bands were amplified including 289 polymarphic bands, counting for 99.01%. Genetic similarity analysis showed F. syreitschikowii germplasm resources of Xinjiang province with high genetic diversity. Through the statistical software analysis, the average number of effective alleles was 1.725 6, the average Nei's genetic diversity index was 0.4048, the average Shannon's information index was 0.587 9, and the genetic similarity coefficients among tested clones ranged from 0.500 0 to 0.828 7. These 96 germplasm resources were divided into two groups and 13 subgroups by UPGMA analysis, which could distinguish the germplasm resources from different sources. Conclusion From the molecular level, it reveals the relationship between the geographical distribution of F. syreitschikowii and the germplasm resources and higher genetic diversity of germplasm resources, which provids basis for identification of F. syreitschikowii varieties.
[中图分类号]
[基金项目]
国家自然科学基金项目(81460574);石河子大学应用基础研究青年项目资助(2015ZRKXYQ16)