目的 研究小花吴茱萸Euodia rutaecarpa var. officinalis的化学成分和抗菌活性。方法 采用各种柱色谱及制备液相等方法进行分离纯化，根据理化性质和波谱数据鉴定化合物的结构，利用菌丝生长法进行抗菌活性的初步筛选。结果 从小花吴茱萸70%乙醇提取物中分离得到15个成分，分别鉴定为吴茱萸碱（1）、吴茱萸次碱（2）、去氢吴茱萸碱（3）、二氢吴茱萸卡品碱（4）、吴茱萸卡品碱（5）、1-甲基-2-十一烷基-4（1H）-喹诺酮（6）、1-甲基-2-[（4Z，7Z）-4，7-十三碳二烯]-4（1H）-喹诺酮（7）、1-甲基-2-[（6Z，9Z）-6，9-十五碳二烯]-4（1H）-喹诺酮（8）、柠檬苦素（9）、石虎柠檬素A（10）、isolimonexic acid（11）、wuzhuyurutine B（12）、豆甾醇（13）、β-谷甾醇（14）、β-胡萝卜苷（15）。结论 化合物5～8和11～13是首次从该植物中得到，不同类型化合物在10 μg/mL质量浓度下对植物病原真菌表现出选择性抑菌作用。

Objective To study the chemical constituents from the fruits of Evodia rutaecarpa var. officinalis. Methods The constituents were isolated and purified by chromatographic methods. The structures were elucidated by physicochemical properties and spectroscopic methods. The antifungal activities were tested with mycelium growth method. Results Fifteen compounds were obtained from the 70% ethanol extract of E. rutaecarpa var. officinalis, and were identified as evodiamine (1), rutaecarpine (2), dehydroevodiamine (3), dihydroevocarpine (4), evocarpine (5), 1-methyl-2-undecyl-4 (1H)-quinolone (6), 1-methyl-2-[(4Z,7Z)- 4,7-tridecadienyl]-4 (1H)-quinolone (7), 1-methyl-2-[(6Z,9Z)-6,9-pentadecadienyl-4 (1H)-quinolone (8), limonin (9), shihulimonin A (10), isolimonexic acid (11), wuzhuyurutine B (12), stigmasterol (13), β-sitosterol (14), and β-daucosterin. (15). Conclusion Compounds 5—8 and 11—13 are isolated from E. rutaecarpa var. officinalis for the first time. Different types of compounds show diverse antimicrobial activities against plant-pathogenic fungi.

国家自然科学基金面上项目（31570359，31470425）；江苏省科技计划项目（省属公益类科研院所能力提升）（BM2015019）