目的 建立一种快速溶剂萃取（ASE）-电雾式检测器（CAD）同时测定酸枣仁中酸枣仁皂苷A、酸枣仁皂苷B、斯皮诺素、白桦脂酸的方法。方法 采用正交试验对快速溶剂萃取系统的提取条件进行优化，并用电雾式检测器对酸枣仁中目标成分进行同时检测。色谱柱为Thermo Syncronis C₁₈（100 mm×3 mm，3 μm），流动相为乙腈-水，体积流量为0.5 mL/min，柱温为40 ℃，Corona Ultra电雾式检测器，雾化温度35 ℃。结果 优化了ASE参数，极大地提高了提取效率，通过1次进样，同时测定酸枣仁中酸枣仁皂苷A、酸枣仁皂苷B、斯皮诺素、白桦脂酸的量，线性关系良好，相关系数为0.998 3～0.999 6，加样回收率在98.46%～102.02%。结论 该方法快速、简便、准确可靠，可用于酸枣仁药材的质量控制。

Objective To establish an accelerated solvent extraction (ASE)-charged aerosol detector (CAD) method for simultaneous detection of jujuboside A, jujuboside B, spinosin, and betulinic acid in Zizyphi Spinosae Semen. Methods The orthogonal design was applied to optimize the extraction parameters of the ASE system. The target compounds were detected by HPLC-CAD with the parameters as follow: Thermo Syncronis C₁₈ (100 mm×3 mm, 3 μm) column, a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 0.5 mL/min, the column temperature was kept at 40 ℃. Detector was Corona Ultra CAD with 35 ℃ of nebulization temperature. Results Optimization of the ASE parameters with orthogonal design greatly improved the extraction efficiency;All the target compounds could be simultaneously determined in a single run. Good linear relationships (0.998 3—0.999 6) and high relative recoveries were 98.46%—102.02%. Conclusion The method is rapid, simple, accurate, and thus could be used for the quality control of Zizyphi Spinosae Semen.