目的 建立菏泽地区丹凤牡丹花瓣的HPLC指纹图谱，并将其应用于不同品种牡丹花的质量评价，同时测定不同品种牡丹花中8种成分的量，为科学评价牡丹花质量提供依据。方法 采用纳微Nano-Micro C₁₈（250 mm×4.6 mm，5 μm）色谱柱；以乙腈-0.1%甲酸水溶液为流动相，梯度洗脱，体积流量1.0 mL/min，检测波长270 nm，柱温25℃。运用中药色谱指纹图谱相似度评价系统进行分析。结果 6批次丹凤牡丹花瓣样品得到的指纹图谱相似度大于0.993，标定共有峰14个，并对其中8个色谱峰进行了归属。同时对6批不同品种牡丹样品与丹凤牡丹进行相似度分析比较，整体相似度较高。同时对牡丹花瓣中没食子酸、没食子酸甲酯、山柰酚-3-O-β-D-吡喃葡萄糖苷（紫云英苷）、芹菜素-7-O-β-D-吡喃葡萄糖苷（大波斯菊苷）、野漆树苷、二氢山柰酚-7-O-β-D-吡喃葡萄糖苷、芍药苷、氧化芍药苷8种主要代谢产物进行了定量测定，其质量分数分别在0.05~1.41、1.01~4.41、0.70~5.55、4.60~18.30、10.05~33.87、0.09~1.76、3.16~11.12、0.85~2.02 mg/g。结论 HPLC指纹图谱结合定量测定能全面反映内在质量，该方法简便可行、快速准确，为牡丹花质量评价提供参考。

Objective To establish an HPLC digital fingerprint for flower petals of Danfeng (Paeonia ostii), and apply it in the determination of other six varieties of P. suffruticosa produced in Heze areas, while determining the contents of eight components in the flower petals. Methods Nano-Micro C₁₈ (250 mm×4.6 mm, 5 μm) column was adopted and the gradient mobile phase consisted of acetonitrile (A)-0.1% formic acid (B) with a flow rate of 1.0 mL/min, the detective wavelength was 270 nm, and the column temperature was 25℃. Results The chromatographic fingerprint similarity evaluation system for traditional Chinese medicines was used for analysis. The chromatographic fingerprint similarity of six batches of Danfeng samples was more than 0.993. There were altogether 14 common peaks, and eight of them were identified. A comparative analysis showed a similarity between samples of Danfeng and other six varieties produced in Heze. Meanwhile, the content of eight major chemical constituents, namely gallic acid, methyl gallate, kaempferol-3-O-β-D-glucopyranoside, apigenin-7-O-β-D-glucopyranoside, apigenin-7-O-β-D-neospheroside, dihydrokaempferol-7-O-β-D-glucopyranoside, paeoniflorin, and oxypaeoniflorin were determined in the range of 0.05-1.41, 1.01-4.41, 0.70-5.55, 4.60-18.30, 10.05-33.87, 0.09-1.76, 3.16-11.12, 0.85-2.02 mg/g. Conclusion The combination of HPLC digital fingerprint and content determination could reflect its inherent quality in an all-round way, which provided scientific basis for the quality evaluation of P. suffruticosa.

山东省科学院青年基金项目（青基合字2015第2号）；山东省自然科学基金三院联合基金项目（ZR2015YL012）