目的 建立HPLC-MS法同时测定知母中新芒果苷、芒果苷、知母皂苷E₁、知母皂苷BⅡ、知母皂苷BⅢ、知母皂苷I、知母皂苷AⅡ、知母皂苷AⅢ 8种化学成分量的方法，分析炮制前后各成分量的变化。方法 色谱柱为Agilent SB-C₁₈柱（50 mm×2.1 mm，1.8 μm），流动相为0.1%甲酸水溶液-乙腈，梯度洗脱，体积流量0.4 mL/min，柱温30℃，进样量10 μL。质谱采用大气压电喷雾离子源，选择性负离子模式监测。结果 所测定的8种成分在各自的线性范围内均具有良好的线性关系（r ≥0.999 7）；平均回收率在97.55%~103.01%，RSD<2.80%。不同产地知母8种成分的量差异较大，其中芒果苷、知母皂苷BⅡ、知母皂苷AⅢ的量较高。知母盐炙后新芒果苷、芒果苷、知母皂苷AⅡ、知母皂苷AⅢ量变化不明显，知母皂苷E₁、知母皂苷BⅡ、知母皂苷I量下降，知母皂苷BⅢ量上升。结论 该方法简单、灵敏、准确，知母盐炙前后多种成分量发生变化。

Objective To establish an HPLC-MS method for the simultaneous quantitation of neomangiferin, mangiferin, timosaponin E₁, timosaponin BⅡ, timosaponin BⅢ, anemarrhenasaponin I, timosaponin AⅢ, and timosaponin AⅡ in Anemarrhenae Rhizoma, and to analyze the content change before and after salt processing. Methods Chromatography was performed on Agilent SB-C₁₈ (50 mm×2.1 mm, 1.8 μm) column by gradient elution using 0.05% formic acid in water (A)-acetonitrile (B) at the flow rate of 0.4 mL/min. The column temperature was kept at 30℃ and the inject volume was 10 μL. HPLC-MS was used for quantification in negative ion mode under ESI. Results The eight components had good linearity (r ≥ 0.9997) within the linear range. The average recovery rate was 97.55%-103.01% with RSD < 2.80%. The contents of the eight components in Anemarrhenae Rhizoma varied according to the different habits. The contents of mangiferin, timosaponin BⅡ, and timosaponin AⅢ were high. After salt processing, contents of timosaponin E₁, timosaponin BⅡ, and anemarrhenasaponin I were reduced, content of timosaponin BⅢ was increased and contents of neomangiferin, mangiferin, timosaponin AⅢ, and timosaponin AⅡ had no obvious changes. Conclusion The changes of components in Anemarrhenae Rhizoma before and after salt processing are proved by the method of HPLC-MS which is simple, sensitive, and accurate.

国家自然科学基金资助项目（81303223）；高等学校博士学科点专项科研基金（20133237120007）；江苏省高校自然科学研究项目（13KJB360012）；江苏省高校优势学科建设工程项目