[关键词]
[摘要]
目的 研究大花益母草Leonurus macranthus的化学成分。方法 采用硅胶、Sephadex LH-20、ODS柱色谱和半制备HPLC等色谱技术进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果 从大花益母草地上部分70%丙酮提取物的二氯甲烷萃取部位分离鉴定了19个化合物,分别鉴定为(+)-丁香脂素(1)、(+)-1-羟基丁香脂素(2)、rayalinol(3)、erythro-guaiacylglycerol-β-O-4'-coniferyl ether(4)、(7R,7'R,7"S,8S,8'S,8"S)-3',4"-dihydroxy-3,5,4',5"-tetramethoxy-7,9':7',9-diepoxy-4,8"-oxy-8,8'-sesquineo-lignan-7",9"-diol(5)、(7R,7'R,7"S,8S,8'S,8"S)-4',5"-dihydroxy-3,5,3',4"-tetramethoxy-7,9':7',9-diepoxy-4,8"-oxy-8,8'-sesquineo-lignan-7",9"-diol(6)、芫花素(7)、3'-羟基芫花素(8)、圣草酚(9)、异莨菪亭(10)、对香豆酸(11)、咖啡酸甲酯(12)、反式-阿魏酸(13)、丁香醛(14)、香草酸(15)、oct-1-en-3-yl β-glucopyranoside(16)、5-羟基-2-吡咯烷酮(17)、pterolactam(18)和烟酰胺(19)。结论 化合物1~6和9~19为首次从益母草属植物中分离得到,化合物7和8为首次从大花益母草中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents from the aerial parts of Leonurus macranthus. Methods The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and semi-preparative HPLC. Their structures were determined by physicochemical properties and spectroscopic data. Results Nineteen compounds were isolated from the CH2Cl2 layer of 70% aqueous acetone extract in the aerial parts of L.macranthus, and identified as (+)-syringaresinol (1), (+)-1-hydroxysyringaresinol (2), rayalinol (3), erythro-guaiacylglycerol-β-O-4'-coniferyl ether (4), (7R,7'R,7"S,8S,8'S,8"S)-3',4"-dihydroxy-3,5,4',5"-tetramethoxy-7,9':7',9-diepoxy-4,8"-oxy-8,8'-sesquineo-lignan-7",9"-diol (5), (7R,7'R,7"S,8S,8'S,8"S)-4',5"-dihydroxy-3,5,3',4"-tetramethoxy-7,9':7',9-diepoxy-4,8"-oxy-8,8'-sesquineo-lignan-7",9"-diol (6), genkwanin (7), 3'-hydroxy-genkwanin (8), eriodictyol (9), isoscopoletin (10), p-coumaric acid (11), caffeic acid methyl ester (12), trans-ferulic acid (13), syringic aldehyde (14), vanillic acid (15), oct-1-en-3-yl β-glucopyranoside (16), 5-hydroxy-2-pyrrolidone (17), pterolactam (18), and nicotinamide (19), respectively. Conclusion Compounds 1-6 and 9-19 are isolated from the plants of genus Leonurus Linn. for the first time, and compounds 7 and 8 are found from L.macranthus for the first time.
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[基金项目]
教育部新世纪优秀人才支持计划资助项目(NCET-13-0693);国家“重大新药创制”科技重大专项资助项目(2013ZX09402201001)