[关键词]
[摘要]
目的 筛选出适合唇形科常见药用植物的DNA条形码序列。方法 通过对33种唇形科常见药用植物的核糖体ITS序列和40种唇形科常见药用植物的叶绿体matK基因进行PCR扩增和测序,用MEGA 6.0软件计算其种间、种内的Kimura 2-parameter(K2P)距离及各序列变异位点,评估序列的条形码间距(barcoding gap),采用邻接法(neighbor-joining,NJ)构建系统聚类树。结果 ITS序列长度为620~698 bp,平均(G+C)量为62.8%,叶绿体matK基因序列长度为859~932 bp,平均(G+C)量为34%,ITS序列与matK基因都有明显的barcoding gap,但matK基因的barcoding gap要小于ITS序列,从聚类分析来看,matK基因能更好地鉴定唇形科不同物种。结论 推荐matK基因序列作为唇形科植物鉴定的优选序列之一。
[Key word]
[Abstract]
Objective To screen a proper DNA barcoding suitable for common medicinal plants in Lamiaceae. Methods The rDNA ITS sequence from 33 kinds of common medicinal plants in Lamiaceae and the chloroplast matK gene sequence from 40 kinds of common medicinal plants in Lamiaceae were amplified by PCR and sequenced. The interspecific and intraspecific Kimura 2-parameter distance (K2P) and the mutation sites in each sequence were calculated, and barcoding gap of sequence was evaluated by MEGA6.0. The phylogenetic trees were constructed on the basis of Neighbor-Joining method. Results The total length of ITS sequence was 620-698 bp, the average G+C content was 62.8%, the chloroplast matK gene sequence length was 859-932 bp, the average G+C content was 34%, and the ITS sequence and matK gene sequence have obvious DNA barcoding gap, but the matK DNA barcoding gap was less than that of ITS gene sequence. From clustering analysis, the matK gene can identify the different species in Lamiaceae better than ITS sequence. Conclusion matK sequence can be used as a preferred sequence to identify the species in Lamiaceae.
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[基金项目]
贵州省社会发展攻关课题(201303137);中央高校基本业务费一般项目(XDJK2016C006)