[关键词]
[摘要]
目的 以活血和抗凝血活性为指标,对复方地龙胶囊质量标准进行再评价研究。方法 采用纤维蛋白平板法,以尿激酶和凝血酶作为对照,分别测定复方地龙胶囊的抗凝血和活血活性。结果 尿激酶在200~1 000 U/mL与透明圈面积呈良好线性关系,r=0.999;凝血酶在8~40 U/mL与沉淀圈面积呈良好线性关系,r=0.997。暂时规定复方地龙胶囊的抗凝血和活血活性分别不低于12 840 U/g和113 822 U/g。结论 本法可以快速、简便、准确地测定复方地龙胶囊的抗凝血和活血活性,可用于复方地龙胶囊的质量控制。
[Key word]
[Abstract]
Objective The quality standard of Compound LumbricalCapsule (CLC) was re-evaluated using blood and anti-coagulation intensity as indexes. Methods Fibrin plate method was used to determine the strength of promoting blood circulation and anti-coagulation for CLC by selecting urokinase and thrombin as reference substances. Results The results of urokinase concentration and transparent circle area showed a good linear relationship in 200-1 000 U/mL, r=0.999; The results of thrombin concentration and precipitation circle area showed a good linear relationship in 8-40 U/mL, r=0.997. The blood anti-coagulant activity of compound earthworm capsule of temporary regulations were not less than 12 840 U/g and 113 822 U/g. Conclusion The method is rapid, simple, and accurate for determining the activity of promoting blood circulation and anticoagulation for compound lumbrical capsule, thus it can be used for the quality control of CLC.
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[基金项目]
四川省千人计划(2013332)