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[摘要]
目的 研究没药Myrrha的化学成分及其对前列腺癌细胞的抑制作用。方法 采用重结晶,硅胶、Sephadex LH-20、开放ODS等柱色谱和制备HPLC方法进行分离纯化,通过理化性质和波谱数据分析进行结构鉴定;采用MTT法,对化合物进行体外抑制前列腺癌细胞PC-3增殖的活性筛选。结果 从没药的三氯甲烷提取物中分离得到11个化合物,分别鉴定为(4α,11α)-2-酮基-8,11-二羟基杜松-1(6),7,9-三烯-12-酸-γ-内酯(1)、(4α,11β)-2-酮基-8,11-二羟基杜松-1(6),7,9-三烯-12-酸-γ-内酯(2)、二氢焦莪术酮(3)、泽泻萜醇E(4)、愈创木烷二醇(5)、柳杉二醇(6)、环阿尔廷-1α,2α,3β,25-四醇(7)、环阿尔廷-24-烯-1α,2α,3β-三醇(8)、环阿尔廷-24-烯-1α,3β-二醇(9)、29-降羊毛脂-8,24-二烯-1α,2α,3β-三醇(10)、十八烷-1,2S,3S,4R-四醇-1-O-α-L-吡喃鼠李糖苷(11)。结论 化合物1、2为新化合物,分别命名为(+)-没药内酯A和(-)-没药内酯A,化合物4、6为首次从没药属植物中分离得到;化合物8、10和11对人前列腺癌PC-3细胞增殖具有中等强度的抑制作用。
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[Abstract]
Objective To study the chemical constituents of Myrrha and their antitumor activities. Methods The constituents were isolated and purified by recrystallization, and open silica gel, Sephadex LH-20, ODS column chromatography, as well as preparative HPLC. The structures were elucidated based on the chemical and spectroscopic methods. Furthermore, the cytotoxicities of these chemical components against PC-3 cell lines were measured by MTT method. Results Eleven compounds were obtained from the chloroform extract of myrrh, and were established as (4α,11α)-2-oxo-8,11-dihydroxycadina-1(6),7,9-trien-12-oic acid γ-lactone (1), (4α,11β)-2-oxo-8,11-dihydroxycadina-1(6),7,9-trien-12-oic acid γ-lactone (2), dihydropyrocurzerenone (3), orientalol E (4), guaianediol (5), cryptomeridiol (6), cycloartane-1α,2α,3β,25-tetrol (7), cycloartan-24-ene-1α,2α,3β-triol (8), cycloartan-24-ene-1α,3β-diol (9), 29-norlanost-8,24-dien-1α,2α,3β-triol (10), and octadecane-1,2S,3S,4R-tetrol-1-O-α-L-rhamnopyranoside (11). Conclusion Compounds 1 and 2 are two new compounds named as (+)-myrrhalactone A and (-)-myrrhalactone A, respectively. Compounds 4 and 6 are isolated from the genus Commiphora Engl. for the first time. Compounds 8, 10, and 11 show moderate cyctotoxic activity against PC-3 cell lines.
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