[关键词]
[摘要]
目的 建立姜黄一测多评测定方法,同时测定其中3种姜黄素类成分量,并进行方法学考察。方法 采用高效液相色谱法,ChromstarTM C18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-4%冰醋酸溶液(48:52),检测波长为422 nm,体积流量为1.0 mL/min,柱温为30℃;以姜黄素为参照物,建立其与去甲氧基姜黄素、双去甲氧基姜黄素相对校正因子,分别以外标法和一测多评法计算3种姜黄素类成分质量分数。结果 该方法可同时测定姜黄中3种姜黄素类成分量,且测定结果与外标法相比无显著性差异(RSD<2.0%)。结论 本法简便、可行、重复性好,可为进一步完善姜黄的质量标准提供参考。
[Key word]
[Abstract]
Objective To establish a quality evaluation method for Curcuma longa, quantitative of multi-component with a single-marker (QAMS), to determine the contents of three curcuminoids at the same time. Methods An HPLC method was used, and a ChromstarTM C18 column (4.6 mm×250 mm, 5 μm) was used. The mobile phase was methanol-4% glacial acetic acid (48︰52) at a flow of 1.0 mL/min. The detection wavelength was 422 nm and column temperature was 30℃. Curcumin was used as the internal reference substance. The relative correlation factors of curcumin, desmethoxycurumin, and bisdesmethoxycurumin to curcumin were calculated and evaluated by standard curve method and QAMS. Results The QAMS method could be used to determine the contents of three curcuminoids at the same time, and with no significant difference between the results of standard curve method (RSD<2.0%). Conclusion QAMS method is simple, feasible, and reproducible, which could provide a reference for further research on completing the quality control of raw material and processed C. longa.
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[基金项目]
四川省科技支撑计划(2014SZ0075,2015SZ0031)