[关键词]
[摘要]
目的 研究大青山灵芝Ganoderma daiqingshanense的化学成分。方法 采用硅胶柱色谱和Sephadex LH-20凝胶柱色谱等技术进行分离纯化,运用各种波谱方法对化合物进行结构鉴定,并测定部分化合物对乙酰胆碱酯酶(AchE)的抑制活性。结果 从大青山灵芝中分离鉴定了12个化合物,经鉴定为3β,21β-山芝烯二醇-3-乙酰基(1)、3β,21α-山芝烯二醇-3-乙酰基(2)、3-O-乙酰基千层塔三醇(3)、20(29)-羽扇烯-3-醇(4)、赤芝醛A(5)、灵芝酸Y(6)、麦角甾-7,22E-二烯-3-酮(7)、麦角甾-4,6,8(14),22E-四烯-3-酮(8)、麦角甾-7,22E-二烯-3β-醇(9)、5α,8α-过氧麦角甾-6,22E-二烯-3β-醇(10)、甲龙胆酸(11)、大戟因子L3(12)。化合物5、11和12对乙酰胆碱酯酶的抑制率分别为17.70%、22.89%和21.22%。结论 所有化合物均为首次从大青山灵芝中分离得到,其中serratene型三萜(化合物1~3)为首次从灵芝科真菌中发现,化合物5、11和12对AchE具有一定的抑制活性。
[Key word]
[Abstract]
Objective To study the chemical constituents of the fruiting bodies of Ganoderma daiqingshanense. Methods The constituents were separated by Silica gel and Sephadex LH-20 column chromatography, their structures were elucidated by spectral data analyses, and the acetylcholinesterase inhibitory activity was detected. Results Twelve compounds were isolated from the fruiting bodies of G. daiqingshanense and identified as 3β,21β-serratenediol-3-acetate (1), 3β,21α-serratenediol-3-acetate (2), 3-O-acetyltohogenol (3), 20 (29)-lupen-3β-ol (4), lucialdehyde A (5), ganoderic acid Y (6), ergosta-7,22E-diene-3-one (7), ergosta-4,6,8 (14),22-tetraene-3-one (8), ergosta-7,22E-diene-3β-ol (9), 5α,8α-epidioxyergosta-6,22E-diene-3β-ol (10), ankylosaurus acid (11), and euphorbia factor L3 (12). Compounds 5, 11, and 12 showed moderate activities against acetylcholinesterase with inhibitory rates of 17.70%, 22.89%, and 21.22%, respectively. Conclusion All the compounds are obtained from G. daiqingshanense for the first time and serratene triterpenes (compounds 1-3) are found from family Ganodermataceae for the first time. Compounds 5, 11, and 12 have certain inhibitory activities on acetylcholinesterase.
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[基金项目]
海南省自然科学基金(20152035);公益性行业(农业)科研专项(201303117);中央级公益性科研院所基本科研业务费专项(ITBB2017)