目的 分离香鳞毛蕨Dryopteris fragrans的间苯三酚类化合物并探讨其抗真菌活性。方法 采用硅胶柱色谱、Sephadex LH-20凝胶和制备HPLC等技术进行化学成分分离，根据理化性质和波谱数据鉴定化合物结构。采用美国临床实验室标准研究所（CLSI）制定的M38-A2方案（微量稀释法）研究化合物对红色毛癣菌和石膏样小孢子菌的抗真菌活性。结果 从香鳞毛蕨中分离得到1对间苯三酚类同分异构体异黄绵马酸PB（1）、黄绵马酸PB（2）。化合物1和2对红色毛癣菌的最低抑菌浓度（MIC）分别为13.33 μg/mL和1.875 μg/mL，对石膏样小孢子菌的MIC分别为10 μg/mL和1.325 μg/mL。结论 化合物1为新化合物，命名为异黄绵马酸PB。化合物2首次从该植物中分离得到。化合物1和2互为同分异构体，且对红色毛癣菌和石膏样小孢子菌均有较好的抑制作用。

Objective To study the chemical constituents and antifungal activitiy of phloroglucinol derivatives from Dryopteris fragrans. Methods The phloroglucinol derivatives were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography, and pre-HPLC. The chemical structures were elucidated on the basis of physicochemical and spectral properties. The antifungal activities against Trichophyton rubrum and Microsporum gypseum were tested by CLSI M38-A2 broth microdilution methods. Results Two phloroglucinol derivatives were isolated and identified as disflavaspidic acid PB (1) and flavaspidic acid PB (2). The minimum inhibitory concentration (MIC) of compounds 1 and 2 were 13.33 and 1.875 μg/mL against T. rubrum respectively, 10 and 1.325 μg/mL against M. gypseum. Conclusion Compound 1 is a new compound named disflavaspidic acid PB. Compound 2 is isolated from D. fragrans for the first time. Compounds 1 and 2 are a pair of isomers with promising antifungal activity.

广东省应用型科技研发专项（2015B020234009）