[关键词]
[摘要]
目的 比较不同种质金线莲中氨基酸和矿物质元素的差异。方法 采用全自动氨基酸分析仪和原子吸收光谱仪测定11个不同种质金线莲中氨基酸和矿物质元素的量。结果 不同种质金线莲必需氨基酸为2.81%~4.47%,总氨基酸量为11.38%~17.06%,其中天门冬氨酸、谷氨酸和精氨酸的量明显高于其他氨基酸组分。矿物质元素中钾元素的量最高,其顺序为K > Ca > Mg > Fe > Mn > Zn > Cr > Cu > Pb > Cd。主成分分析表明丙氨酸、丝氨酸、谷氨酸、天门冬氨酸、苏氨酸、异亮氨酸、丙氨酸、苯丙氨酸、亮氨酸为金线莲的特征性氨基酸,Fe、Zn、Mn、Ca、Cr、Mg为金线莲的特征性元素。结论 金线莲中氨基酸和矿物质元素量存在地域性差异。
[Key word]
[Abstract]
Objective To compare the contents of mineral elements and amino acids in different germplasms of Anoectochilus roxburghii. Methods The contents of amino acids and mineral elements in 11 different germplasms of A. roxburghii were determined by automatic amino acid analyzer and atomic absorption spectrometer. Results The range of essential amino acids in the different species of A. roxburghii was 2.81%-4.47%, and the range of total amino acids was 11.38%-17.06%. Aspartic acid, glutamic acid, and lysine contents were significantly higher than those of other amino acids. The content of potassium was the highest, and the order was:K > Ca > Mg > Fe > Mn > Zn > Cr > Cu > Pb > Cd. The principal components analysis showed that alanine, serine, glutamic acid, aspartic acid, threonine, isoleucine, alanine, phenylalanine, and leucine were characteristic amino acids in A. roxburghii. Fe, Zn, Mn, Ca, Cr, and Mg were characteristic elements of A. roxburghii. Conclusion There are regional differences of A. roxburghii between amino acids and the content of mineral elements.
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[基金项目]
国家自然科学基金项目(81303167,81673531);浙江省农业新品种选育重大科技专项(2016C02058);浙江省大学生科技创新活动(新苗人才)计划基金项目