目的 研究刺五加内生放线菌株13-85抑菌活性及活性稳定性，检测菌株活性物质合成相关功能基因，对菌株进行鉴定。方法 采用平板纸片法测定菌株抑菌活性及发酵液稳定性；PCR扩增其活性物质合成相关功能基因片段（PKS I、PKS Ⅱ、NRPS、Halo、CYP），克隆并测序分析。通过菌株表型特征及分子特征对其鉴定。结果 菌株13-85发酵液具有广谱较强的抗病原菌活性，抑菌活性较为稳定；菌株基因组含有PKS Ⅱ、NRPS基因。经比对，PKS Ⅱ片段与已知Streptomyces fradiae PKS序列（登录号AFO70128.1）相似度为89%，NRPS片段与Streptomyces virginiae NRPS序列（登录号WP_033225509.1）相似度达95%。菌株13-85与Streptomyces amritsarensis（登录号GQ906975）16 S rRNA基因序列相似率高达99.65%。结论 菌株13-85发酵液有较为广泛的抑菌活性且稳定性较好，菌株含有PKS Ⅱ、NRPS基因，初步鉴定该放线菌为链霉菌属有效发表种Streptomyces amritsarensis的一个菌株，具进一步开发应用价值。

Objective To studies the antimicrobial activity and stability of fermentation extracts produced by endophytic actinomycete strain 13-85 from Acanthopanax senticosus; Secondary metabolite biosynthesis genes were detected. Meanwhile, the strain was identified. Methods Paper disc diffusion method was used to test the antimicrobial activity and stability of strain 13-85, the biosynthesis genes(PKS I, PKS Ⅱ, NRPS, Halo, and CYP) were obtained by PCR, and sequences were analyzed. The strain was identified by phenotypic characteristics and molecular characteristics. Results The fermentation broth of the strain showed broad-spectrum, strong activity, and stability against pathogenic microbes. The strain contains PKS Ⅱ gene and NRPS gene. The PKS Ⅱ sequence had 89% homology compared with Streptomyces fradiae PKS gene(Accession Number:AFO70128.1). The NRPS gene sequence had 95% homology with S. virginiae NRPS gene(Accession Number:WP_033225509.1). The sequence of 16S rRNA of the strain had 99.85% homology with S. amritsarensis(Accession Number:GQ906975). Conclusion Metabolites of the actinomycete strain 13-85 with broad-spectrum antibacterial activity and better stability, have a value for development and application. The strain 13-85 was identified as Streptomyces amritsarensis, a valid species of genus Streptomyces.

河北省自然科学基金资助项目（C2014209137）