目的 探讨山楂降三酰甘油（TG）主要活性成分UPLC指纹图谱与药效之间的相关性。方法 利用TG体外模拟消化模型，筛选出山楂降TG主要活性组分。采用UPLC法建立12批不同产地山楂降TG主要活性组分指纹图谱，并获取相应的药效数据，运用偏最小二乘法，进行相关性分析。结果 山楂中富含黄酮的组分能够明显地降低TG水平。经相关性分析，在该组分指纹图谱所含的11个共有峰中，1（B型原花青素二聚体）、3（原花青素C1）、4（B型原花青素二聚体）、5（芦丁）、6（金丝桃苷）、7（异槲皮苷）、8（B型原花青素二聚体）号色谱峰所对应的化合物对降TG呈正相关作用，其余包括2（表儿茶素）、10（槲皮素）、11（5-O-咖啡奎尼丁酯）号色谱峰在内的各峰则呈负相关。结论 山楂黄酮类成分对降低TG水平存在不同程度的影响。

Objective To investigate the correlation between UPLC fingerprints of triglyceride(TG)-lowering fraction of Crataegus pinnatifida fruit and its efficacy. Methods In vitro digestion model for TG was applied to screen the TG-lowering fraction of C. pinnatifida fruit. The fingerprints of TG-lowering fraction of 12 batches of hawthorn fruits from different habitats were established by UPLC. And the partial least squares regression(PLSR) analysis was used to explore the correlation between peak areas of UPLC fingerprints and TG content. Results Flavonoids rich fraction of C. pinnatifida fruit exhibited stronger TG decreasing activity than other fractions. Eleven common peaks of the active fraction were selected. Peaks 1(procyanidins dimers B), 3(procyanidins C1), 4(procyanidins dimers B), 5(rutin), 6(hyperoside), 7(isoquercitrin), and 8(procyanidins dimers B) had positive effect on TG decreasing activity, while peaks 2(epicatechin), 10(quercitin), and 11(5-O-caffeoyl quinic acid butyl ester) had negative effect. Conclusion The TG-lowering effect is closely related to flavonoids.

国家自然科学基金资助项目（81260638）；江西省自然科学基金项目（20161BAB205220）