目的 通过研究大黄-栀子配伍比例和提取工艺对大黄蒽醌类成分溶出的影响，从分子角度诠释大黄-栀子药对在不同方剂中的组方依据。方法 采用水煎法和超声醇提法提取不同配比的大黄-栀子药对中大黄蒽醌类成分，通过HPLC法同时测定总蒽醌和游离蒽醌量，基于总蒽醌为游离蒽醌和结合型蒽醌之和，计算出结合型蒽醌量。结果 水煎法中大黄配伍栀子后各类型蒽醌量增加，其中结合型蒽醌量明显增加，说明配伍栀子有助于结合态蒽醌溶出。水煎法中，大黄先下、后下、同时下等提取工艺也会对蒽醌类成分溶出产生影响。超声法中，分提方式利于总蒽醌和游离蒽醌的溶出；合提方式利于结合性蒽醌的溶出。超声法提取效率明显高于水煎法。结论 不同配比的大黄-栀子药对因其蒽醌类成分组成与量不同而在不同方剂中发挥着不同的作用，从而说明了经典组方的合理性。

Objective To investigate the impact of compatibility ratios of rhubarb-gardenia herbal pair and extraction techniques on anthraquinone dissolution, and to explain the bases of different compatibility of rhubarb-gardenia in the prescriptions of Chinese materia medica from chemical composition. Methods Anthraquinones were extracted from rhubarb-gardenia herbal pair of different compatibility ratios by water decoction method and ultrasonic extraction with alcohol. Free and total anthraquinones were analyzed by HPLC and the contents of binding anthraquinones were equal to the total minus the free anthraquinones. Results The contents of various types of anthraquinones extracted by water decoction method increased and binding anthraquinone content increased significantly after rhubarb was compatible with gardenia. This suggested that gardenia was benefit for binding anthraquinone dissolution. Decocting order(Rhubarb cooked first, last, and at the same time) also affected the content of anthraquinones in water decoction method. Separate ultrasonic extraction was benefit for total and free anthraquinone dissolution. Separate ultrasonic extraction was benefit for total and free anthraquinones dissolution. Simultaneous ultrasonic extraction was benefit for binding anthraquinone dissolution. The extraction effect of ultrasonic method was significantly higher than that of water decoction method. Conclusion Rhubarb-gardenia herbal pair in different compatibility proportion plays different role in different prescriptions because of the different composition and content of anthraquinones, which proves the rationality of the classic formula.

国家自科基金面上项目（81173533）；湖南省教育厅项目（15C0118）；长沙市科技局项目（K1509005-31）