目的 建立广地龙药材中化学成分的超高液相色谱-质谱联用（UPLC-Q-TOF-MS）分析方法。方法 采用Waters ACQUITY UPLC BEH C₁₈（100 mm×2.1 mm，1.7μm）色谱柱，以0.1%甲酸水-0.1%甲酸乙腈为流动相梯度洗脱，体积流量0.3 mL/min，柱温35℃，正负离子模式扫描。结果 共鉴定出广地龙药材中84个化学成分，包括11种游离氨基酸、26种有机酸类、11种核苷类、5种二肽及环二肽类、21种含氮类物质和10种其他类。结论 该法准确、快速、灵敏，为进一步阐明其药效物质基础和后期质量控制指标的选择提供科学依据。

Objective To establish a method for the analysis of constituents of Pheretima aspergillum by UPLC-Q-TOF-MS. Methods The analysis was performed on an Acquity UPLC BEH C₁₈ column(100 mm×2.1 mm, 1.7 μm) by gradient elution. The mobile phase consist of 0.1% formic acid-acetonitrile and 0.1% formic acid-water at a flow rate of 0.3 mL/min. The column temperature was at 35℃. The information of the compounds was acquired in positive and negative mode. Results Total 83 compounds were inferred, contains 11 free amino acids, 26 organic acids, 11 necleosides, 5 dipeptides and cyclic dipeptideds and other 21 nitrogen-containing substances, the rest on a total of 10. Conclusion The method is accurate, rapid, and sensitive, and provide a basis for further clarifying the material basis of its efficacy and the selection of quality control indicators.

国家自然科学基金项目（81377405）；黑龙江省青年基金项目（QC2011C114）