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[摘要]
目的 研究龙胆科龙胆属小秦艽Gentiana dahurica干燥花的化学成分。方法 采用硅胶、Sephadex LH-20、MCI、反相ODS等色谱材料进行分离纯化,通过理化性质和波谱数据鉴定化合物的结构。结果 从小秦艽花的乙醇提取物中分离得到20个化合物,包括8个三萜类成分:栎瘿酸(1)、3β-乙酰氧基-28-羟基-12-烯-齐墩果烷(2)、28-羟基-α-香树脂醇(3)、28-羟基-β-香树脂醇(4)、α-香树脂醇(5)、β-香树脂醇(6)、熊果酸(7)、齐墩果酸(8);12个黄酮类成分:1-羟基-3,7,8-三甲氧基酮(9)、山柰酚(10)、柚皮素(11)、芹菜素(12)、木犀草素(13)、(2S)-5,4'-二羟基-7-O-β-D-葡萄糖-2,3-二氢黄酮(14)、芹菜素-7-O-β-D-吡喃葡萄糖苷(15)、木犀草素-7-O-β-D-吡喃葡萄糖苷(16)、木犀草素-6-C-β-D-吡喃葡萄糖苷(17)、芹菜素-6-C-β-D-吡喃葡萄糖苷(18)、芹菜素-6-C-β-D-葡萄糖-7-O-β-D-葡萄糖苷(19)、木犀草素-6-C-β-D-葡萄糖-7-O-β-D-葡萄糖苷(20)。结论 化合物1~11、13~16、19、20均为首次从小秦艽花中分离得到;化合物2~7、9~11、13~16、19、20均为首次从该植物中分离得到。
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[Abstract]
Objective To investigate the chemical constituents of the flowers of Gentiana dahurica. Methods All compounds were isolated and purified by silica gel, Sephadex LH-20, ODS, and MCI column chromatography. Their structures were determined by physicochemical properties and spectral data. Results Twenty compounds were isolated from the flowers of G. dahurica. Among them, eight triterpenoids were identified as roburic acid(1), 3β-acetoxy-28-hydroxy-12-ene-oleanane(2), 28-hydroxy-α-amyrin(3), 28-hydroxy-β-amyrin(4), α-amyrin(5), β-amyrin(6), ursolic acid(7), and oleanolic acid(8). Twelve flavonoids were identified as 1-hydroxy-3, 7, 8-trimethoxyxanthone(9), kaempferol(10), naringenin(11), apigenin(12), luteolin(13),(2S)-naringenin-7-O-β-D-glucopyranoside(14), apigenin-7-O-β-D-glucopyranoside(15), luteolin-7-O-β-D-glucopyranoside(16), isoorientin(17), isovitexin(18), saponarin(19), and lutonarin(20). Conclusion Seventeen compounds 1-11, 13-16, 19, and 20 are found from flowers of G. dahurica for the first time; Compounds 2-7, 9-11, 13-16, 19, and 20 are isolated from this species for the first time.
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