目的 利用¹H-NMR为基础的代谢组学研究牛黄解毒片中人工牛黄、石膏、冰片3味中药对雄黄的配伍减毒作用。方法 42只大鼠随机分成6组：A组（对照组）、B组（雄黄组）、C组（牛黄解毒片组）、D组（牛黄解毒片去人工牛黄组）、E组（牛黄解毒片去石膏组）、F组（牛黄解毒片去冰片组）。对各组大鼠尿液及血清样品¹H-NMR谱图进行模式识别分析。结果 雄黄组大鼠样品代谢轮廓和对照组差异明显，其他各组大鼠代谢轮廓同对照组相似，向对照组回归。结论 牛黄解毒片中人工牛黄、石膏、冰片3味中药对雄黄配伍减毒作用不明显。

Objective To study the toxicity alleviation effect of Bovis Calculus Artificialis, Gypsum Fibrosum, and Borneolum Synthcticum to Realgar in Niuhuang Jiedu Tablet based on ¹H-NMR metabonomics. Methods Forty-two male Wistar rats were divided into six groups:group A (control group), group B (treated with realgar), group C (treated with Niuhuang Jiedu Tablet), group D (treated with Niuhuang Jiedu Tablet without Bovis Calculus Artificialis), group E (treated with Niuhuang Jiedu Tablet without Gypsum Fibrosum), and group F (treated with Niuhuang Jiedu Tablet without Borneolum Synthcticum). Based on ¹H-NMR spectra of urine and serum from rats, PLS-DA was performed to identify different metabolic profiles. Results The metabolic profiles of group B were different from control group, while the metabolic profiles of all other groups were almost in line with those of control group. Conclusion The toxicity alleviation effects of Bovis Calculus Artificialis, Gypsum Fibrosum, and Borneolum Synthcticum to Realgar in Niuhuang Jiedu Tablet are not obvious.

国家自然科学基金资助项目（81073035）