目的 利用超高效液相色谱配置二极管阵列检测器，旨在建立同时测定黑果腺肋花楸浆果中原花青素B1、原花青素B2、原花青素B4、芦丁和槲皮素的方法。方法 色谱柱为ACQUITY UPLC^® HSS T3 C₁₈色谱柱（100 mm×2.1 mm，1.8 μm）；甲醇-0.03%磷酸水溶液为流动相，梯度洗脱；柱温35℃；体积流量为0.2 mL/min；检测波长：原花青素B1、原花青素B2和原花青素B4为280 nm，芦丁和槲皮素为360 nm。结果 原花青素B1、原花青素B2、原花青素B4、芦丁和槲皮素分别在2.2～44.0 μg/mL（r=0.999 2）、50～1 000 μg/mL（r=0.999 5）、3.6～54.0 μg/mL（r=0.999 2）、7.4～148.0 μg/mL（r=0.999 4）和2.1～42.0 μg/mL（r=0.999 7）呈良好的线性关系，平均回收率分别为101.23%、99.64%、102.31%、98.74%和103.53%，RSD分别为2.13%、1.23%、2.89%、2.96%、2.64%。黑果腺肋花楸浆果中原花青素B1、原花青素B2、原花青素B4、芦丁和槲皮素的平均质量分数分别为129.78、3 834.99、196.02、134.40、79.10 μg/g。结论 该方法操作简单、结果准确，具有较好的重复性和稳定性，可用于同时测定黑果腺肋花楸浆果中5种主要有效成分，将为黑果腺肋花楸质量控制提供科学依据。

Objective The ultra performance liquid chromatography diode array detection method was used to establish the determination methods for the contents of procyanidin B1, procyanidin B2, procyanidin B4, rutin, and quercetin in Aronia melanocarpa berry. Methods Waters Acquity UPLC^® HSS T3 C₁₈ column (100 mm×2.1 mm, 1.8 μm) was used. The mobile phase was methanol (A)-0.03% phosphoric acid in water (B) for the gradient elution with flow rate of 0.2 mL/min. The column temperature was 35℃. The determination wavelength for procyanidin B1, procyanidin B2 and procyanidin B4 was 280 nm, and that for rutin and quercetin was 360 nm. Results There was a linear correlation between the concentration of procyanidin B1 2.2-44.0 μg/mL (r=0.999 2), procyanidin B2 50-1 000 μg/mL (r=0.999 5), procyanidin B4 3.6-54.0 μg/mL (r=0.999 2), rutin 7.4-148.0 μg/mL (r=0.999 4), and quercetin 2.1-42.0 μg/mL (r=0.999 7). The average recovery rates of procyanidin B1, procyanidin B2, procyanidin B4, rutin, and quercetin were 101.23% (RSD=2.13%), 99.64% (RSD=1.23%), 102.31% (RSD=2.89%), 98.74% (RSD=2.96%), and 103.53% (RSD=2.64%), respectively. The average contents of procyanidin B1, procyanidin B2, procyanidin B4, rutin, and quercetin in Aronia melanocarpa berry were 129.78, 3 834.99, 196.02, 134.40 and 79.10 μg/g, respectively. Conclusion The analysis method is simple, rapid, reproducible, accurate, and reliable, and can be used to identify and evaluate the quantitative determination of five constituents in A. melanocarpa berry.

黑龙江省应用技术研究与开发计划项目（GC13C106）；黑龙江省自然科学基金资助（H2016057）；黑龙江中医药大学“优秀创新人才支持计划”项目（2012）；2016年哈尔滨商业大学青年创新人才支持项目（2016QN076）